Printed covalent glycan array for ligand profiling of diverse glycan binding proteins

Ola Blixt; Steve Head; Tony Mondala; Christopher Scanlan; Margaret E. Huflejt; Richard Alvarez; Marian C. Bryan; Fabio Fazio; Daniel Calarese; James Stevens; Nahid Razi; David J. Stevens; John J. Skehel; Irma van Die; Dennis R. Burton; Ian A. Wilson

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Printed covalent glycan array for ligand profiling of diverse glycan binding proteins

【24h】

Printed covalent glycan array for ligand profiling of diverse glycan binding proteins

机译：印刷的共价聚糖阵列，用于各种聚糖结合蛋白的配体分析

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Here we describe a glycan microarray constructed by using standard robotic microarray printing technology to couple amine func-tionalized glycans to an amino-reactive glass slide. The array comprises 200 synthetic and natural glycan sequences representing major glycan structures of glycoproteins and glycolipids. The array has remarkable utility for profiling the specificity of a diverse range of glycan binding proteins, including C-type lectins, siglecs, galec-tins, anticarbohydrate antibodies, lectins from plants and microbes, and intact viruses.

机译：在这里，我们描述了通过使用标准的机器人微阵列印刷技术将胺功能化的聚糖与氨基反应性载玻片偶联而构建的聚糖微阵列。该阵列包含代表糖蛋白和糖脂的主要聚糖结构的200个合成和天然聚糖序列。该阵列在分析多种聚糖结合蛋白（包括C型凝集素，siglecs，galec-tins，抗碳水化合物抗体，植物和微生物的凝集素以及完整病毒）的特异性方面具有显着的实用性。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2004年第49期|p.17033-17038|共6页
作者
Ola Blixt; Steve Head; Tony Mondala; Christopher Scanlan; Margaret E. Huflejt; Richard Alvarez; Marian C. Bryan; Fabio Fazio; Daniel Calarese; James Stevens; Nahid Razi; David J. Stevens; John J. Skehel; Irma van Die; Dennis R. Burton; Ian A. Wilson;
展开▼
作者单位

Glycan Synthesis and Protein Expression Core-D, Consortium for Functional Glycomics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
carbohydrate; lectin; microarray; glycoprotein; glycolipid;

机译：碳水化合物;凝集素;微阵列;糖蛋白;糖脂;

相似文献

外文文献
中文文献
专利

1. Structural insights into what glycan arrays tell us about how glycan-binding proteins interact with their ligands. [J] . Taylor ME, Drickamer K Glycobiology. . 2009,第11期

机译：关于哪些聚糖阵列的结构洞察力告诉我们有关聚糖结合蛋白如何与其配体相互作用的信息。
2. Structural insights into what glycan arrays tell us about how glycan-binding proteins interact with their ligands [J] . Maureen E Taylor and Kurt Drickamer1 Glycobiology . 2009,第11期

机译：关于聚糖阵列的结构洞察力告诉我们聚糖结合蛋白如何与其配体相互作用
3. Cell-based glycan arrays for probing glycan–glycan binding protein interactions [J] . Jennie Grace Briard, Hao Jiang, Kelley W. Moremen, Nature Communications . 2018,第1期

机译：基于细胞的聚糖阵列，用于探测聚糖与聚糖结合蛋白的相互作用
4. COVALENT GLYCAN MICRO ARRAY AND BIACORE CHIPS FOR STUDYING LYSM PROTEIN BINDING TO CHITIN, PEPTIDOGLYCAN AND NOD FACTORS [C] . Maolanon. N.N., Blaise M., Sorensen K.K. International Carbohydrate Symposium . 2013

机译：共价甘油微阵列和Biacore芯片用于研究与甲壳素，肽聚糖和点头因子结合的Lysm蛋白
5. A systems approach to analyzing bacterial glycans and glycan-binding proteins [D] . Hsu, Ku-Lung 2008

机译：分析细菌聚糖和聚糖结合蛋白的系统方法
6. Printed covalent glycan array for ligand profiling of diverse glycan binding proteins [O] . Ola Blixt, Steve Head, Tony Mondala, 2004

机译：印刷的共价聚糖阵列用于各种聚糖结合蛋白的配体分析
7. Printed covalent glycan array for ligand profiling of diverse glycan binding proteins [O] . Blixt, Ola, Head, Steve, Mondala, Tony, 2004

机译：印刷的共价聚糖阵列，用于各种聚糖结合蛋白的配体分析

Printed covalent glycan array for ligand profiling of diverse glycan binding proteins

摘要

著录项

相似文献

相关主题

期刊订阅