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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Radial glia give rise to adult neural stem cells in the subventricular zone.
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Radial glia give rise to adult neural stem cells in the subventricular zone.

机译：ial神经胶质细胞会在脑室下区域产生成年的神经干细胞。

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摘要

Neural stem cells with the characteristics of astrocytes persist in the subventricular zone (SVZ) of the juvenile and adult brain. These cells generate large numbers of new neurons that migrate through the rostral migratory stream to the olfactory bulb. The developmental origin of adult neural stem cells is not known. Here, we describe a lox-Cre-based technique to specifically and permanently label a restricted population of striatal radial glia in newborn mice. Within the first few days after labeling, these radial glial cells gave rise to neurons, oligodendrocytes, and astrocytes, including astrocytes in the SVZ. Remarkably, the rostral migratory stream contained labeled migratory neuroblasts at all ages examined, including 150-day-old mice. Labeling dividing cells with the S-phase marker BrdUrd showed that new neurons continue to be produced in the adult by precursors ultimately derived from radial glia. Furthermore, both radial glia in neonates and radial glia-derived cells in the adult lateral ventricular wall generated self-renewing, multipotent neurospheres. These results demonstrate that radial glial cells not only serve as progenitors for many neurons and glial cells soon after birth but also give rise to adult SVZ stem cells that continue to produce neurons throughout adult life. This study identifies and provides a method to genetically modify the lineage that links neonatal and adult neural stem cells.
机译：具有星形胶质细胞特征的神经干细胞在少年和成年大脑的脑室下区域（SVZ）中持续存在。这些细胞产生大量新的神经元，这些神经元通过鼻部迁徙流迁移到嗅球。成人神经干细胞的发育起源尚不清楚。在这里，我们描述了一种基于lox-Cre的技术，可以专门和永久地标记新生小鼠中纹状体放射状胶质细胞的受限制群体。在标记后的头几天内，这些放射状神经胶质细胞产生了神经元，少突胶质细胞和星形胶质细胞，包括SVZ中的星形胶质细胞。值得注意的是，在所有检查的各个年龄段（包括150日龄的小鼠），鼻端迁徙流都包含标记的迁徙成神经细胞。用S期标记BrdUrd标记分裂细胞表明，成年后最终从radial神经胶质细胞衍生的前体继续产生新的神经元。此外，新生儿的放射状胶质细胞和成年的侧脑室壁的放射状胶质细胞均产生了自我更新的，多能的神经球。这些结果表明，放射状神经胶质细胞不仅在出生后立即成为许多神经元和神经胶质细胞的祖细胞，而且还产生了成年SVZ干细胞，这些成年SVZ干细胞会在整个成年后继续产生神经元。这项研究鉴定并提供了一种遗传方法来遗传修饰连接新生儿和成年神经干细胞的谱系。

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