Diffusion of nucleoside triphosphates and role of the entry site to the RNA polymerase II active center.

Batada NN; Westover KD; Bushnell DA; Levitt M; Kornberg RD

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Diffusion of nucleoside triphosphates and role of the entry site to the RNA polymerase II active center.

机译：核苷三磷酸的扩散和RNA聚合酶II活性中心进入位点的作用。

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摘要

Nucleoside triphosphates (NTPs) diffuse to the active center of RNA polymerase II through a funnel-shaped opening that narrows to a negatively charged pore. Computer simulation shows that the funnel and pore reduce the rate of diffusion by a factor of approximately 2 x 10(-7). The resulting limitation on the rate of RNA synthesis under conditions of low NTP concentration may be overcome by NTP binding to an entry site adjacent to the active center. Binding to the entry site greatly enhances the lifetime of an NTP in the active center region, and it prevents backtracking

机译：核苷三磷酸酯（NTPs）通过一个漏斗形的开口扩散到RNA聚合酶II的活性中心，该开口变窄为带负电荷的孔。计算机仿真表明，漏斗和孔将扩散速率降低了大约2 x 10（-7）。在低NTP浓度条件下，RNA合成速率的最终限制可以通过NTP结合到与活性中心相邻的进入位点来克服。绑定到入口站点可以大大延长活动中心区域中NTP的寿命，并防止回溯

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2004年第50期|P.17361-17364|共4页
作者
Batada NN; Westover KD; Bushnell DA; Levitt M; Kornberg RD;
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作者单位

Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305-5126, USA.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
DNA-Directed RNA Polymerase; Diffusion; nucleoside triphosphate; binding; 扩散;

机译：DNA指导的RNA聚合酶;扩散;三磷酸核苷;结合;扩散;

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专利

1. Human RNA Polymerase II Elongation in Slow Motion: Role of the TFIIF RAP74 α1 Helix in Nucleoside Triphosphate-Driven Translocation [J] . Chunfen Zhang, Katie L. Zobeck, Zachary F. Burton Molecular and Cellular Biology . 2005,第9期

机译：人类RNA聚合酶II延长慢动作：TFIIF RAP74α1螺旋在核苷三磷酸酯驱动的移位中的作用
2. Transcription Factors IIF and IIS and Nucleoside Triphosphate Substrates as Dynamic Probes of the Human RNA Polymerase II Mechanism. [J] . Zhang C, Burton ZF Journal of Molecular Biology . 2004,第4期

机译：转录因子IIF和IIS和核苷三磷酸底物作为人类RNA聚合酶II机制的动态探针。
3. Transcription factors IIF and US and nucleoside triphosphate substrates as dynamic probes of the human RNA polymerase II mechanism [J] . Zhang CF, Burton ZF Journal of Molecular Biology . 2004,第4期

机译：转录因子IIF和US和三磷酸核苷底物作为人类RNA聚合酶II机制的动态探针
4. Determination of highly polar, active nucleoside triphosphate in biological matrices using a multi-mode HPLC approach coupled with mass spectrometry [C] . A. Liu, E. Fung, A. Aubry, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：使用多模HPLC方法与质谱法耦合的生物基质中高极性，活性核苷三磷酸三磷酸盐
5. Nonpolar nucleoside isosteres as probes of polymerase active sites. [D] . Helquist, Sandra A. 2006

机译：非极性核苷等位基因作为聚合酶活性位点的探针。
6. Diffusion of nucleoside triphosphates and role of the entry site to the RNA polymerase II active center [O] . Nizar N. Batada, Kenneth D. Westover, David A. Bushnell, 2004

机译：核苷三磷酸的扩散和RNA聚合酶II活性中心进入位点的作用
7. Diffusion of nucleoside triphosphates and role of the entry site to the RNA polymerase II active center [O] . Batada, Nizar N., Westover, Kenneth D., Bushnell, David A., 2004

机译：核苷三磷酸的扩散和RNA聚合酶II活性中心进入位点的作用

Diffusion of nucleoside triphosphates and role of the entry site to the RNA polymerase II active center.

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