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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The bacteriophage T4 late-transcription coactivator gp33 binds the flap domain of Escherichia coli RNA polymerase.
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The bacteriophage T4 late-transcription coactivator gp33 binds the flap domain of Escherichia coli RNA polymerase.

机译：噬菌体T4晚期转录共激活因子gp33结合大肠杆菌RNA聚合酶的襟翼结构域。

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摘要

Transcription of bacteriophage T4 late genes requires concomitant DNA replication. T4 late promoters, which consist of a single 8-bp -10 motif, are recognized by a holoenzyme containing Escherichia coli RNA polymerase core and the T4-encoded promoter specificity subunit, gp55. Initiation of transcription at these promoters by gp55-holoenzyme is inefficient, but is greatly activated by the DNA-loaded DNA polymerase sliding clamp, gp45, and the coactivator, gp33. We report that gp33 attaches to the flap domain of the Escherichia coli RNA polymerase beta-subunit and that this interaction is essential for activation. The beta-flap also mediates recognition of -35 promoter motifs by binding to sigma(70) domain 4. The results suggest that gp33 is an analogue of sigma(70) domain 4 and that gp55 and gp33 together constitute two parts of the T4 late sigma. We propose a model for the role of the gp45 sliding clamp in activation of T4 late-gene transcription.
机译：噬菌体T4晚期基因的转录需要伴随的DNA复制。 T4晚期启动子由单个8 bp -10母题组成，被含有大肠杆菌RNA聚合酶核心和T4编码的启动子特异性亚基gp55的全酶识别。 gp55-全酶在这些启动子上的转录启动效率低下，但是被载有DNA的DNA聚合酶滑动夹gp45和共激活因子gp33极大地激活了。我们报告gp33附加到大肠杆菌RNA聚合酶β亚基的皮瓣域，并且这种相互作用对于激活至关重要。 β襟翼还通过与sigma（70）域4结合来介导-35启动子基序的识别。结果表明gp33是sigma（70）域4的类似物，并且gp55和gp33一起构成T4晚期的两个部分西格玛我们提出了gp45滑动夹具在T4晚期基因转录激活中的作用模型。

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