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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission
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Canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission

机译：信号传递不需要拟南芥ETR1乙烯受体的递质结构域的典型组氨酸激酶活性

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摘要

Ethylene signaling in plants is mediated by a family of receptors related to bacterial two-component histidine kinases. Of the five members of the Arabidopsis ethylene receptor family, members of subfamily I (ETR1 and ERS1) contain completely conserved histidine kinase domains, whereas members of subfamily II (ETR2, EIN4, and ERS2) lack conserved residues thought to be necessary for kinase activity. To examine the role of the conserved histidine kinase domain in receptor signaling, ers1;etr1 loss-of-function double mutants were generated. The double mutants exhibited a severe constitutive ethylene response phenotype consistent with the negative regulator model for receptor function. The adult err1-2,etr1-6 and ers1-2,etr1-7 phenotypes included miniature rosette size, delayed flowering, and both male and female sterility, whereas etiolated-seedling responses were less affected. Chimeric transgene constructs in which the ETR1 promoter was used to drive expression of cDNAs for each of the five receptor isoforms were transferred into the ers1-2,etr1-7double-mutant plants. Subfamily I constructs restored normal growth, whereas subfamily II constructs failed to rescue the double mutant, providing evidence for a unique role for subfamily I in receptor signaling. However, transformation of either the ers1-2,etr1-6 or ers1-2,etr1-7 mutant with a kinase-in activated ETR1 genomic clone also resulted in complete restoration of normal growth and ethylene responsiveness in the double-mutant background, leading to the conclusion that canonical histidine kinase activity by receptors is not required for ethylene receptor signaling. [References: 35]
机译：植物中的乙烯信号传导是由与细菌两组分组氨酸激酶相关的一系列受体介导的。在拟南芥乙烯受体家族的五个成员中，亚家族I的成员（ETR1和ERS1）包含完全保守的组氨酸激酶结构域，而亚家族II的成员（ETR2，EIN4和ERS2）缺少保守的残基，这些残基被认为是激酶活性所必需的。为了检查保守的组氨酸激酶结构域在受体信号传导中的作用，产生了ers1; etr1功能丧失的双突变体。双重突变体表现出严重的本构乙烯反应表型，与受体功能的负调节剂模型一致。成人err1-2，etr1-6和ers1-2，etr1-7表型包括微型莲座丛大小，延迟开花以及雄性和雌性不育，而黄化苗期反应的影响较小。将其中ETR1启动子用于驱动cDNA表达的嵌合转基因构建体，这五个受体同工型中的每一个均被转移到ers1-2，etr1-7双突变植物中。亚家族I的构建体恢复了正常的生长，而亚家族II的构建体未能挽救双重突变体，为亚家族I在受体信号传导中的独特作用提供了证据。但是，用激酶激活的ETR1基因组克隆转化ers1-2，etr1-6或ers1-2，etr1-7突变体也能在双突变背景下完全恢复正常生长和乙烯反应，导致得出的结论是，乙烯受体信号传导不需要受体的典型组氨酸激酶活性。 [参考：35]

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