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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Experimental test of a method for determining causal connectivities of species in reactions.
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Experimental test of a method for determining causal connectivities of species in reactions.

机译：确定反应中物种因果连通性的方法的实验测试。

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Theoretical analysis has shown the possibility of determining causal connectivities of reacting species and the reaction mechanism in complex chemical and biochemical reaction systems by applying pulse changes of concentrations of one or more species, of arbitrary magnitude, and measuring the temporal response of as many species as possible. This method, limited to measured and pulsed species, is given here an experimental test on a part of glycolysis including the sequence of reactions from glucose to fructose 1,6-biphosphate, followed by the bifurcation of that sequence into two branches, one ending in glycerol 3-phosphate, the other in glyceraldehyde 3-phosphate. Pulses of concentrations of one species at a time are applied to the open system in a non-equilibrium stationary state, and the temporal responses in concentrations of six metabolites are measured by capillary zone electrophoresis. From the results of these measurements and the use of the theory for their interpretation, we establish the causal connectivities of the metabolites and thus the reaction mechanism, including the bifurcation of one chain of reactions into two. In this test case of the pulse method, no prior knowledge was assumed of the biochemistry of this system. We conclude that the pulse method is relatively simple and effective in determining reaction mechanisms in complex systems, including reactants, products, intermediates, and catalysts and their effectors. The method is likely to be useful for substantially more complex systems.
机译：理论分析表明，可以通过施加任意数量的一种或多种浓度的脉冲变化，并测量与最大数量相同的物种的时间响应，来确定复杂的化学和生化反应系统中反应物种的因果联系以及反应机理。可能。此方法仅限于测量和脉冲的物质，此处对一部分糖酵解进行了实验测试，包括从葡萄糖到果糖1,6-二磷酸的反应顺序，然后将该顺序分为两个分支，一个终止于甘油3-磷酸，另一个在甘油3-磷酸。一次将一种物质的浓度脉冲以非平衡固定状态应用于开放系统，并通过毛细管区带电泳测量六种代谢物浓度的时间响应。从这些测量的结果以及使用理论进行解释，我们确定了代谢物的因果联系，从而建立了反应机理，包括将一个反应链分成两部分。在脉冲方法的这个测试案例中，没有假定对该系统的生物化学有先验知识。我们得出的结论是，脉冲法在确定复杂系统（包括反应物，产物，中间体和催化剂及其效应器）中的反应机理方面相对简单有效。该方法可能对于实质上更复杂的系统很有用。

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