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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Structural origins of adenine-tract bending.
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Structural origins of adenine-tract bending.

机译：腺嘌呤弯曲的结构起源。

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摘要

DNA sequences containing short adenine tracts are intrinsically curved and play a role in transcriptional regulation. Despite many high-resolution NMR and x-ray studies, the origins of curvature remain disputed. Long-range restraints provided by 85 residual dipolar couplings were measured for a DNA decamer containing an adenine (A)(4)-tract and used to refine the structure. The overall bend in the molecule is a result of in-phase negative roll in the A-tract and positive roll at its 5' junction, as well as positive and negative tilt inside the A-tract and near its junctions. The bend magnitude and direction obtained from NMR structures is 9.0 degrees into the minor groove in a coordinate frame located at the third AT base pair. We evaluated long-range and wedge models for DNA curvature and concluded that our data for A-tract curvature are best explained by a "delocalized bend" model. The global bend magnitude and direction of the NMR structure are in excellent agreement with the junction model parameters used to rationalize gel electrophoretic data and with preliminary results of a cyclization kinetics assay from our laboratory.
机译：含有短腺嘌呤段的DNA序列在本质上是弯曲的，并在转录调控中起作用。尽管进行了许多高分辨率的NMR和X射线研究，但曲率的起源仍然存在争议。测量了含有腺嘌呤（A）（4）片段的DNA decamer的85个残留偶极偶合提供的远程约束，并用于完善结构。分子的整体弯曲是由于A区域中的同相负侧倾和其5'接点处的正侧倾以及A区域内部及其接点附近的正负倾斜引起的。从NMR结构获得的弯曲幅度和方向与位于第三AT碱基对处的坐标框中的小凹槽成9.0度。我们评估了DNA曲率的远程和楔形模型，并得出结论，我们的A束曲率数据最好用“离域弯曲”模型来解释。 NMR结构的整体弯曲幅度和方向与用于合理化凝胶电泳数据的连接模型参数以及来自我们实验室的环化动力学测定的初步结果极好一致。

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