...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Kinetics of size changes of individual Bacillus thuringiensis spores in response to changes in relative humidity.
【24h】

Kinetics of size changes of individual Bacillus thuringiensis spores in response to changes in relative humidity.

机译：苏云金芽孢杆菌单个孢子相对于相对湿度变化的大小变化动力学。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using an automated scanning microscope, we report the surprising result that individual dormant spores of Bacillus thuringiensis grow and shrink in response to increasing and decreasing relative humidity. We simultaneously monitored the size of inorganic calibration particles. We found that the spores consistently swell in response to increased relative humidity, and shrink to near their original size on reexposure to dry air. Although the dispersion of swelling amplitudes within an ensemble of spores is wide (approximately 30% of the average amplitude), amplitudes for individual spores are highly correlated between different swelling episodes, suggesting that individual spores respond consistently to changes in humidity. We find evidence for two distinct time scales for swelling: one with a time scale of no more than approximately 50 s, and another with a time scale of approximately 8 min. We speculate that these two mechanisms may be due to rapid diffusion of water into the spore coat + cortex, followed by slower diffusion of water into the spore core, respectively. Humidity-dependent swelling may account for the greater kill effectiveness of spores by gas-phase chlorine dioxide, formaldehyde, and ethylene oxide at very high relative humidity.
机译：使用自动扫描显微镜，我们报告了令人惊讶的结果，苏云金芽孢杆菌的单个休眠孢子随着相对湿度的增加和减少而生长和收缩。我们同时监测了无机校准颗粒的大小。我们发现，随着相对湿度的增加，孢子会持续膨胀，再暴露于干燥的空气中会收缩至其原始大小附近。尽管肿胀幅度在一组孢子中的散布范围很广（大约为平均幅度的30％），但单个孢子的幅度在不同的肿胀发作之间高度相关，这表明单个孢子对湿度的变化始终如一地做出反应。我们发现了两个不同的肿胀时间尺度的证据：一个不超过大约50 s的时间尺度，另一个不超过8分钟的时间尺度。我们推测这两种机制可能是由于水迅速扩散到孢子皮层+皮层，然后分别缓慢扩散到了孢子芯。湿度相关的溶胀可解释在很高的相对湿度下气相二氧化氯，甲醛和环氧乙烷对孢子的杀灭作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号