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首页> 外文会议>Pacific Rim conference on biotechnology of bacillus thuringiensis >RAPD Finger-print and Spore Coast Protein of Bacillus thuringiensis and Related Bacillus Species
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RAPD Finger-print and Spore Coast Protein of Bacillus thuringiensis and Related Bacillus Species

机译：芽孢杆菌的RAPD手指印刷和孢子海岸蛋白质和相关芽孢杆菌物种

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摘要

Randomly amplified Polymorphic DNA (RAPD) fingerprint and spore coat protein in Bacillus thuringiensis and related Bacillus species were investigated. The homogeneity of H-antigen was investigated simultaneously. It was showed that there were no homogeneity of H-antigen in 5 Bacillus species. From 6 arbitrary primers the primer NP2 was the only one which was able to produce PCR products in all tested species. The RAPD fingerprints of various species were different, and those of various subspecies and strains were different also. The SDS-PAGE patterns of spore coat proteins in 5 species showed a distinguished difference, but there were a partial homogeneity between B. thuringiensis and B. cereus, and high similarity in different subspecies of B. thuringiensis. It was proposed that the SDS-PAGE patterns of spore coat protein could be used as a important item for the taxonomy of genus Bacillus and B. thuringiensis.
机译：研究了芽孢杆菌和相关杆菌物种中随机扩增的多态DNA（RAPD）指纹和孢子涂层蛋白。同时研究了H-抗原的均匀性。结果表明，在5个杆菌物种中没有H-Antigen的均匀性。来自6个任意引物，引物NP2是唯一一个能够在所有测试物种中生产PCR产物的引物。各种物种的RAPD指纹不同，各种亚种和菌株的菌株也不同。 5种物种中孢子壳蛋白的SDS-PAGE模式表现出具有杰出差异，但B.trauringiensis和B.培养物之间存在部分均匀性，B.培训群和B.培斯芽的不同亚种性的高相似性。建议孢子涂层蛋白的SDS-PAGE模式可用作芽孢杆菌和B. Thuringiensis属的分类学的重要项目。

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