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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression).
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Phage HK022 Nun protein represses translation of phage lambda N (transcription termination/translation repression).

机译：噬菌体HK022 Nun蛋白可抑制噬菌体λN的翻译（转录终止/翻译抑制）。

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摘要

The N-terminal arginine-rich motif of phage HK022 Nun protein binds to NUT sequences in phage lambda nascent transcripts and induces transcription termination. Interactions between the Nun C terminus and RNA polymerase as well as the DNA template are required for termination. We have isolated Nun C-terminal point and deletion mutants that are unable to block transcription. The mutants bind NUT RNA and inhibit translation of the lambda N gene. Thus HK022 excludes lambda both by terminating transcription on the phage chromosome and by preventing translation of the essential lambda N gene. Like N autoregulation, translation repression by Nun requires host RNaseIII deficiency (rnc) or a mutation in the RNaseIII processing site (rIII) located between NUTL and the beginning of the N coding sequence. Our data support the idea that Nun bound at NUTL causes steric interference with ribosome attachment to the nearby N coding sequence. Two models, Nun acting alone or in complex with host proteins, are discussed.
机译：噬菌体HK022 Nun蛋白的N末端富含精氨酸的基序与噬菌体λ新生转录本中的NUT序列结合并诱导转录终止。终止需要Nun C末端与RNA聚合酶以及DNA模板之间的相互作用。我们已经分离出无法阻断转录的Nun C末端和缺失突变体。突变体结合NUT RNA并抑制λN基因的翻译。因此，HK022通过终止噬菌体染色体上的转录和通过阻止必需的λN基因的翻译而排除了λ。像N自动调节一样，Nun的翻译抑制作用要求宿主RNaseIII缺乏（rnc）或位于NUTL和N编码序列开头之间的RNaseIII加工位点（rIII）发生突变。我们的数据支持这样的想法，即在NUTL处结合的Nun会导致核糖体与附近N个编码序列的连接发生空间干扰。讨论了两种模型，Nun单独起作用或与宿主蛋白共同起作用。

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