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首页> 外文期刊>Journal of Molecular Biology >PHAGE HK022 NUN PROTEIN ARRESTS TRANSCRIPTION ON PHAGE LAMBDA DNA IN VITRO AND COMPETES WITH THE PHAGE LAMBDA N ANTITERMINATION PROTEIN
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PHAGE HK022 NUN PROTEIN ARRESTS TRANSCRIPTION ON PHAGE LAMBDA DNA IN VITRO AND COMPETES WITH THE PHAGE LAMBDA N ANTITERMINATION PROTEIN

机译：噬菌体HK022 Nun蛋白体外转录噬菌体Lambda DNA并与噬菌体Lambda N抗蛋白竞争。

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摘要

Phage HK022 Nun protein excludes phage lambda by terminating transcription near the lambda nut sites. We have established a purified in vitro system that reproduces the in vivo sequence and factor requirements of Nun. Nun arrests transcription by E. coli RNA polymerase at or near elongation pause sites distal to the nut sites. The boxB sequence of nut is required for optimal Nun activity; boxA plays a lesser role. The efficiency of transcription arrest is strongly enhanced by the four E. coli Nus factors. The factors increase the specific activity of Nun, and allow it to act at higher ribonucleoside triphosphate concentrations. A wild-type boxA is required for stimulation by Nus factors. Nun and the lambda N antitermination protein compete for their opposing reactions. This competition may be at the level of binding of boxB RNA. [References: 37]
机译：噬菌体HK022 Nun蛋白通过在λ螺母位点附近终止转录而排除了λ噬菌体。我们建立了一个纯化的体外系统，该系统可重现Nun的体内序列和因子要求。修女在远离坚果位点的延伸暂停位点处或附近阻止大肠杆菌RNA聚合酶的转录。螺母的boxB序列是获得最佳Nun活性所必需的; boxA的作用较小。四种大肠杆菌Nus因子可大大提高转录阻止的效率。这些因素增加了Nun的比活性，并使其在较高的核糖核苷三磷酸浓度下起作用。 Nus因子刺激需要野生​​型boxA。尼姑和λN抗终止蛋白竞争其相反的反应。竞争可能在boxB RNA的结合水平上。 [参考：37]

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