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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ultra-high-pressure inactivation of priori infectivity in processed meat: A practical method to prevent human infection
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Ultra-high-pressure inactivation of priori infectivity in processed meat: A practical method to prevent human infection

机译：超高压灭活加工肉的先验感染性：预防人类感染的实用方法

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Bovine spongiform encephalopathy contamination of the human food chain most likely resulted from nervous system tissue in mechanically recovered meat used in the manufacture of processed meats. We spiked hot dogs with 263K hamster-adapted scrapie brain (10% wt/wt) to produce an infectivity level of ≈9 log_(10) mean lethaf doses (LD_(50)) per g of paste homogenate, Aliquots were subjected to short pressure pulses of 690, 1,000, and 1,200 MPa at running temperatures of 121―137℃. Western blots of PrPres were found to be useful indicators of infectivity levels, which at all tested pressures were significantly reduced as compared with untreated controls: from ≈10~3 LD~(50) per g at 690 MPa to ≈10~6 LD_(50) per g at 1,200 MPa. The application of commercially practical conditions of temperature and pressure could ensure the safety of processed meats from bovine spongiform encephalopathy contamination, and could also be used to study phase transitions of the prion protein from its normal to misfolded state.
机译：牛海绵状脑病对人类食物链的污染最有可能是由在加工肉类生产中机械回收的肉中的神经系统组织引起的。我们用263K适应仓鼠的瘙痒病脑（10％wt / wt）掺入热狗，以产生每克糊状均浆化的≈9 log_（10）平均生菜剂量（LD_（50））的感染水平，将等分试样置于短距离内在121〜137℃的工作温度下，压力脉冲分别为690、1,000和1200 MPa。已发现PrPres的Western印迹是感染性水平的有用指标，在所有测试压力下，与未处理的对照相比，其显着降低：在690 MPa下从≈10〜3 LD〜（50）/ g降低到≈10〜6 LD_（ 50）/ g，1200 MPa。商业上实用的温度和压力条件可以确保加工的肉类免受牛海绵状脑病污染的安全性，还可以用于研究ion病毒蛋白从正常状态到错误折叠状态的相变。

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