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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The two-component PhoR-PhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans
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The two-component PhoR-PhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans

机译：两组分PhoR-PhoP系统可控制青霉链霉菌的主要代谢和次生代谢物的生物合成

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摘要

The biosynthesis of most secondary metabolites in different bacteria is strongly depressed by inorganic phosphate. The two-component phoR-phoP system of Streptomyces lividans has been doned and characterized. PhoR showed all of the characteristics of the membrane-bound sensor proteins, whereas PhoP is a member of the DNA-binding OmpR family. Deletion mutants lacking phoP or phoR-phoP, were unable to grow in minimal medium at low phosphate concentration (10 μM). Growth was fully restored by complementation with the phoR-phoP genes. Both S. lividans ΔphoP and ΔphoR-phoP deletion mutants were unable to synthesize extracellular alkaline phosphatase (AP) as shown by immuno-detection with anti-AP antibodies and by enzymatic analysis, suggesting that the PhoR-PhoP system is required for expression of the AP gene (phoA). Synthesis of AP was restored by complementation of the deletion mutants with phoR-phoP. The biosynthesis of two secondary metabolites, actinorhodin and undecylprodigio-sin, was significantly increased in both solid and liquid medium in the ΔphoP or ΔhoR-phoP deletion mutants. Negative phosphate control of both secondary metabolites was restored by complementation with the phoR-phoP cluster. These results prove that expression of both phoA and genes implicated in the biosynthesis of secondary metabolites in S. lividans is regulated by a mechanism involving the two-component PhoR-PhoP system.
机译：无机磷酸盐强烈抑制了不同细菌中大多数次级代谢产物的生物合成。猪链霉菌的两组分phoR-phoP系统已被使用和表征。 PhoR显示了膜结合传感器蛋白的所有特征，而PhoP是结合DNA的OmpR家族的成员。缺少phoP或phoR-phoP的缺失突变体无法在低磷酸盐浓度（10μM）的基本培养基中生长。通过与phoR-phoP基因互补可完全恢复生长。 lividans的ΔphoP和ΔphoR-phoP缺失突变体均无法合成细胞外碱性磷酸酶（AP），如通过抗AP抗体的免疫检测和酶促分析所表明的那样，表明PhoR-PhoP系统是表达该蛋白所必需的AP基因（phoA）。通过缺失突变体与phoR-phoP互补来恢复AP的合成。在ΔphoP或ΔhoR-phoP缺失突变体的固体和液体培养基中，两种次生代谢产物，放线菌丝蛋白和十一烷基脯氨酸的生物合成均显着增加。通过与phoR-phoP簇互补，恢复了两个次级代谢产物的负磷酸盐控制。这些结果证明，phoA和参与lividans次生代谢产物生物合成的基因的表达均受涉及两组分PhoR-PhoP系统的机制调控。

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