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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect
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Interaction of soft condensed materials with living cells: Phenotype/transcriptome correlations for the hydrophobic effect

机译：软质冷凝物与活细胞的相互作用：疏水作用的表型/转录组相关性

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摘要

The assessment of biomaterial compatibility relies heavily on the analysis of macroscopic cellular responses to material interaction. However, new technologies have become available that permit a more profound understanding of the molecular basis of cell-biomaterial interaction. Here, both conventional phenotypic and contemporary transcriptomic (DNA microarray-based) analysis techniques were combined to examine the interaction of cells with a homologous series of copolymer films that subtly vary in terms of surface hydrophobicity. More specifically, we used differing combinations of N-isopropylacrylamide, which is presently used as an adaptive cell culture substrate, and the more hydrophobic, yet structurally similar, monomer N-tert-butylacrylamide. We show here that even discrete modifications with respect to the physiochemistry of soft amorphous materials can lead to significant impacts on the phenotype of interacting cells. Furthermore, we have elucidated putative links between phenotypic responses to cell-biomaterial interaction and global gene expression profile alterations. This case study indicates that high-throughput analysis of gene expression not only can greatly refine our knowledge of cell-biomaterial interaction, but also can yield novel biomarkers for potential use in biocompatibility assessment. [References: 22]
机译：生物材料相容性的评估在很大程度上取决于对物质相互作用的宏观细胞反应的分析。但是，已经出现了可以使人们对细胞-生物材料相互作用的分子基础有更深刻理解的新技术。在这里，传统的表型和当代的转录组学（基于DNA芯片）分析技术相结合，以检查细胞与一系列同源的共聚物膜之间的相互作用，该膜在表面疏水性方面有所不同。更具体地说，我们使用了N-异丙基丙烯酰胺（目前用作适应性细胞培养底物）和疏水性更强但结构相似的单体N-叔丁基丙烯酰胺的不同组合。我们在这里表明，就软非晶材料的物理化学而言，即使是离散的修饰也可能对相互作用细胞的表型产生重大影响。此外，我们阐明了对细胞-生物材料相互作用的表型反应与全局基因表达谱改变之间的推定联系。该案例研究表明，基因表达的高通量分析不仅可以极大地改善我们对细胞-生物材料相互作用的了解，而且可以产生新的生物标记物，以潜在地用于生物相容性评估。 [参考：22]

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