How the BfiI restriction enzyme uses one active site to cut two DNA strands

Sasnauskas G.; Halford SE.; Siksnys V.

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >How the BfiI restriction enzyme uses one active site to cut two DNA strands

【24h】

How the BfiI restriction enzyme uses one active site to cut two DNA strands

机译：BfiI限制酶如何利用一个活性位点切割两条DNA链

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Unlike other restriction enzymes, BfiI functions without metal ions. It recognizes an asymmetric DNA sequence, 5'-ACTGGG-3', and cuts top and bottom strands at fixed positions downstream of this sequence. Many restriction enzymes are dimers of identical subunits, with one active site for each DNA strand. Others, like FokI, dimerize transiently during catalysis. BfiI is also a dimer but it has only one active site, at the dinner interface. We show here that BfiI remains a dinner as it makes double-strand breaks in DNA and that its single active site acts sequentially, first on the bottom and then the top strand. Hence, after cutting the bottom strand, a rearrangement of either the protein and/or the DNA in the BfiI-DNA complex must switch the active site to the top strand. Low pH values selectively block top-strand cleavage, converting BfiI into a nicking enzyme that cleaves only the bottom strand. The switch to the top strand may depend on the ionization of the cleaved 5' phosphate in the bottom strand. BfiI thus uses a mechanism for making double-strand breaks that is novel among restriction enzymes. [References: 30]

机译：与其他限制酶不同，BfiI在没有金属离子的情况下起作用。它识别不对称的DNA序列5'-ACTGGG-3'，并在该序列下游的固定位置切割上链和下链。许多限制酶是相同亚基的二聚体，每条DNA链都有一个活性位点。其他诸如FokI的催化剂在催化过程中会瞬间二聚。 BfiI也是一个二聚体，但在晚餐界面上只有一个活动位点。我们在这里显示BfiI仍然是一顿晚餐，因为它使DNA发生双链断裂，并且其单个活性位点依次作用于底部，然后依次作用于顶部。因此，在切割底部链之后，BfiI-DNA复合物中蛋白质和/或DNA的重排必须将活性位点切换到顶部链。低pH值会选择性阻断上链切割，从而将BfiI转换为仅切割下链的切刻酶。切换到顶部链可能取决于底部链中切割的5'磷酸的离子化。因此，BfiI使用一种在限制酶中新颖的双链断裂机制。 [参考：30]

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2003年第11期|p. 6410-6415|共6页
作者
Sasnauskas G.; Halford SE.; Siksnys V.;
展开▼
作者单位

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Endonuclease pi-scei; Crystal-structure; I-tevi; Cleavage; Foki; Transposition; Recombination; Recognition; Intermediate; Proteins;

机译：核糖核酸内切酶晶体结构I-tevi;劈裂福基;换位;重组;承认;中间;蛋白质类;

相似文献

外文文献
中文文献
专利

1. A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site [J] . Giedrius Sasnauskas, James W. Gaynor, Linas Zakrys, Nucleic acids research . 2010,第7期

机译：切断双链DNA的新机制：BfiI通过旋转单个活性位点切割3'–5'和5'-3'链
2. The Metal-independent Type IIs Restriction Enzyme BfiI is a Dimer that Binds Two DNA Sites but has Only One Catalytic Centre. [J] . Lagunavicius A, Sasnauskas G, Halford SE, Journal of Molecular Biology . 2003,第4期

机译：不依赖金属的IIs型限制性酶BfiI是一种二聚体，可结合两个DNA位点，但只有一个催化中心。
3. Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme [J] . Golovenko Dmitrij, Manakova Elena, Zakrys Linas, Nucleic Acids Research . 2014,第6期

机译：BfiI限制酶C末端片段的共晶体结构提供的B3 DNA结合域特异性结构的结构见解
4. DNA looping and cleavage by restriction enzymes studied by manipulation of single DNA molecules with optical tweezers [C] . Douglas E. Smith, Gregory J. Gemmen, Rachel Millin Optical Trapping and Optical Micromanipulation III . 2006

机译：用光镊操作单个DNA分子研究限制酶对DNA的环化和切割
5. THE RESTRICTION ENDONUCLEASE ECO RI: ITS INTRINSIC METAL CENTER AND SPECIFIC INTERACTIONS WITH A DNA RECOGNITION SITE (PROTEIN-NUCLEIC ACID, ZINC METALLOENZYMES, INTERACTIONS CHIRAL METAL COMPLEXES, DNA-BINDING PROTEINS). [D] . PARANAWITHANA, SHANTHI R. 1986

机译：限制性内切酶Eco RI：其内在金属中心以及与DNA识别位点的特定相互作用（蛋白-核酸，锌金属酶，相互作用的手性金属络合物，DNA结合蛋白）。
6. How the BfiI restriction enzyme uses one active site to cut two DNA strands [O] . Giedrius Sasnauskas, Stephen E. Halford, Virginijus Siksnys 2003

机译：BfiI限制酶如何利用一个活性位点切割两条DNA链
7. How the BfiI restriction enzyme uses one active site to cut two DNA strands [O] . Sasnauskas, Giedrius, Halford, Stephen E., Siksnys, Virginijus 2003

机译：BfiI限制酶如何利用一个活性位点切割两条DNA链
8. Simple, rapid HLA-DQA1 genotyping using the polymerase chain reaction and analysis by single-strand conformation polymorphism and restriction-enzyme cleavage [R] . Hayashi, T. , Seyama, T. , Ito, T. , 1993

机译：使用聚合酶链反应进行简单，快速的HLa-DQa1基因分型，并通过单链构象多态性和限制酶切割进行分析

How the BfiI restriction enzyme uses one active site to cut two DNA strands

摘要

著录项

相似文献

相关主题

期刊订阅