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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy
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Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy

机译:通过二次谐波显微镜在本地脑组织中成像的均匀极性微管组件

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摘要

Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs. [References: 46]
机译:微管(MT)集合极性是神经元过程的结构和功能的诊断决定因素。在这里,极化的MT结构在自然脑组织中通过二次谐波生成(SHG)显微镜选择性成像。发现该SHG与脑切片和培养的神经元中的轴突共定位。因为SHG仅由同相对称结构产生,所以轴突MT的均匀极性导致观察到的信号,而树突中混合的极性导致相消干涉。 SHG成像提供了一种工具,可用于研究动态天然脑组织结构和其他基于极化MT的亚细胞运动结构中MT集合极性的动力学和功能。 [参考:46]

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