...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: Implication for land plant evolution
【24h】

Comparative genomics of Physcomitrella patens gametophytic transcriptome and Arabidopsis thaliana: Implication for land plant evolution

机译：Physcomitrella patens配子体转录组和拟南芥的比较基因组学：对陆地植物进化的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mosses and flowering plants diverged >400 million years ago. The mosses have haploid-dominant life cycles, whereas the flowering plants are diploid-dominant. The common ancestors of land plants have been inferred to be haploid-dominant, suggesting that genes used in the diploid body of flowering plants were recruited from the genes used in the haploid body of the ancestors during the evolution of land plants. To assess this evolutionary hypothesis, we constructed an EST library of the moss Physcomitrella patens, and compared the moss transcriptome to the genome of Arabidopsis thaliana. We constructed full-length enriched cDNA libraries from auxin-treated, cytokinin-treated, and untreated gametophytes of P. patens, and sequenced both ends of >40,000 clones. These data, together with the mRNA sequences in the public databases, were assembled into 15,883 putative transcripts. Sequence comparisons of A. thaliana and P. patens showed that at least 66% of the A. thaliana genes had homologues in A patens. Comparison of the A patens putative transcripts with all known proteins, revealed 9,907 putative transcripts with high levels of similarity to vascular plant genes, and 850 putative transcripts with high levels of similarity to other organisms. The haploid transcriptome of A patens appears to be quite similar to the A. thaliana genome, supporting the evolutionary hypothesis. Our study also revealed that a number of genes are moss specific and were lost in the flowering plant lineage. [References: 57]
机译：苔藓和开花植物在4亿多年前发生了分歧。苔藓具有单倍体优势的生命周期，而开花植物则以二倍体优势。据推断，陆地植物的共同祖先是单倍体优势的，这表明在陆地植物的进化过程中，开花植物的二倍体中使用的基因是从祖先的单倍体中使用的基因中募集的。为了评估这一进化假说，我们构建了苔藓小立碗藓的EST库，并将苔藓转录组与拟南芥的基因组进行了比较。我们从生长素处理，细胞分裂素处理和未处理的配子体配子构建了全长富集的cDNA文库，并对> 40,000个克隆的两端进行了测序。这些数据与公共数据库中的mRNA序列一起被组装成15,883个假定的转录本。拟南芥和P. patens的序列比较表明，至少66％的拟南芥基因在A patens中具有同源物。将A patens推定转录本与所有已知蛋白质进行比较，发现与维管植物基因高度相似的9,907个推定转录本，与其他生物高度相似的850个推定转录本。 A patens的单倍体转录组似乎与拟南芥基因组非常相似，从而支持进化假说。我们的研究还表明，许多基因是苔藓特异的，并且在开花植物谱系中丢失。 [参考：57]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号