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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9.
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Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9.

机译：肌管蛋白相关蛋白7及其结合伴侣肌管蛋白相关蛋白9。

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摘要

Myotubularin-related protein 7 (MTMR7) is a member of the myotubularin (MTM) family. The cDNA encoding the mouse MTMR7 contains 1,983 bp, and the predicted protein has a deduced molecular mass of 75.6 kDa. Northern and Western blot analyses showed that MTMR7 is expressed mainly in brain and mouse neuroblastoma N1E-115 cells. Recombinant MTMR7 dephosphorylated the D-3 position of phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate [Ins(1,3)P2]. The substrate specificity of MTMR7 is different than other MTM proteins in that this enzyme prefers the water-soluble substrate. Immunofluorescence showed that MTMR7 is localized in Golgi-like granules and cytosol, and subcellular fractionation showed both cytoplasmic and membrane localization of MTMR7 in N1E-115 cells. An MTMR7-binding protein was found in an anti-MTMR7 immunoprecipitate from N1E-115 cells and identified as MTM-related protein 9 (MTMR9) by tandem mass spectrometry. The coiled-coil domain of MTMR9 was sufficient for binding to MTMR7. The binding of MTMR9 increased the Ins(1,3)P2 phosphatase activity of MTMR7. Our results show that MTMR7 forms a complex with MTMR9 and dephosphorylates phosphatidylinositol 3-phosphate and Ins(1,3)P2 in neuronal cells.
机译：肌管蛋白相关蛋白7（MTMR7）是肌管蛋白（MTM）家族的成员。编码小鼠MTMR7的cDNA包含1,983 bp，预测的蛋白质推导的分子量为75.6 kDa。 Northern和Western印迹分析表明，MTMR7主要在脑和小鼠神经母细胞瘤N1E-115细胞中表达。重组MTMR7使磷脂酰肌醇3-磷酸和肌醇1,3-双磷酸[Ins（1,3）P2]的D-3位置去磷酸化。 MTMR7的底物特异性不同于其他MTM蛋白，因为该酶偏爱水溶性底物。免疫荧光显示MTMR7位于高尔基样颗粒和细胞质中，亚细胞分级显示MTMR7在N1E-115细胞中的胞质和膜定位。在N1E-115细胞的抗MTMR7免疫沉淀物中发现了MTMR7结合蛋白，并通过串联质谱法鉴定为MTM相关蛋白9（MTMR9）。 MTMR9的卷曲螺旋结构域足以结合MTMR7。 MTMR9的结合增加了MTMR7的Ins（1,3）P2磷酸酶活性。我们的结果表明，MTMR7与MTMR9形成复合物，并在神经元细胞中使磷脂酰肌醇3-磷酸和Ins（1,3）P2去磷酸化。

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