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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mpi recombinase globally modulates the surface architecture of a human commensal bacterium.
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Mpi recombinase globally modulates the surface architecture of a human commensal bacterium.

机译：Mpi重组酶总体上调节人类共生细菌的表面结构。

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摘要

The mammalian gut represents a complex and diverse ecosystem, consisting of unique interactions between the host and microbial residents. Bacterial surfaces serve as an interface that promotes and responds to this dynamic exchange, a process essential to the biology of both symbionts. The human intestinal microorganism, Bacteroides fragilis, is able to extensively modulate its surface. Analysis of the B. fragilis genomic sequence, together with genetic conservation analyses, cross-species cloning experiments, and mutational studies, revealed that this organism utilizes an endogenous DNA inversion factor to globally modulate the expression of its surface structures. This DNA invertase is necessary for the inversion of at least 13 regions located throughout the genome, including the promoter regions for seven of the capsular polysaccharide biosynthesis loci, an accessory polysaccharide biosynthesis locus, and five other regions containing consensus promoter sequences. Bacterial DNA invertases of the serine site-specific recombinase family are typically encoded by imported elements such as phage and plasmids, and act locally on a single region of the imported element. In contrast, the conservation and unique global regulatory nature of the process in B. fragilis suggest an evolutionarily ancient mechanism for surface adaptation to the changing intestinal milieu during commensalism.
机译：哺乳动物的肠道代表了一个复杂多样的生态系统，由宿主与微生物居民之间的独特相互作用组成。细菌表面充当促进和响应这种动态交换的界面，这是两个共生体生物学必不可少的过程。人类肠道微生物脆弱拟杆菌（Bacteroides fragilis）能够广泛调节其表面。对脆弱芽孢杆菌基因组序列的分析，以及基因保守性分析，跨物种克隆实验和突变研究表明，该生物利用内源性DNA转化因子来整体调节其表面结构的表达。该DNA转化酶对于转化位于整个基因组中的至少13个区域是必需的，所述区域包括七个荚膜多糖生物合成基因座的启动子区域，一个辅助多糖生物合成基因座以及五个包含共有启动子序列的其他区域。丝氨酸位点特异性重组酶家族的细菌DNA转化酶通常由输入的元件如噬菌体和质粒编码，并局部作用于输入的元件的单个区域。相比之下，脆弱类芽孢杆菌中该过程的保守性和独特的全球调控性质表明，在共鸣过程中，表面适应不断变化的肠道环境的进化古老机制。

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