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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake.
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A tenth atp gene and the conserved atpI gene of a Bacillus atp operon have a role in Mg2+ uptake.

机译：芽孢杆菌atp操纵子的第十个atp基因和保守的atpI基因在摄取Mg2 +中起作用。

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摘要

The atp operon of alkaliphilic Bacillus pseudofirmus OF4, as in most prokaryotes, contains the eight structural genes for the F-ATPase (ATP synthase), which are preceded by an atpI gene that encodes a membrane protein of unknown function. A tenth gene, atpZ, has been found in this operon, which is upstream of and overlapping with atpI. Most Bacillus species, and some other bacteria, possess atpZ homologues. AtpZ is predicted to be a membrane protein with a hairpin topology, and was detected by Western analyses. Deletion of atpZ, atpI, or atpZI from B. pseudofirmus OF4 led to a requirement for a greatly increased concentration of Mg2+ for growth at pH 7.5. Either atpZ, atpI, or atpZI complemented the similar phenotype of a triple mutant of Salmonella typhimurium (MM281), which is deficient in Mg2+ uptake. atpZ and atpI, separately and together, increased the Mg2+-sensitive 45Ca2+ uptake by vesicles of an Escherichia coli mutant that is defective in Ca2+ and Na+ efflux. We hypothesize that AtpZ and AtpI,as homooligomers, and perhaps as heterooligomers, are Mg2+ transporter, Ca2+ transporter, or channel proteins. Such proteins could provide Mg2+, which is required by ATP synthase, and also support charge compensation, when the enzyme is functioning in the hydrolytic direction; e.g., during cytoplasmic pH regulation.
机译：像大多数原核生物一样，嗜碱芽孢杆菌OF4的atp操纵子包含F-ATPase（ATP合酶）的八个结构基因，其后是编码未知功能膜蛋白的atpI基因。在该操纵子中发现了第十个基因atpZ，它位于atpI的上游并与之重叠。大多数芽孢杆菌属物种和其他一些细菌都具有atpZ同源物。 AtpZ被预测为具有发夹形拓扑的膜蛋白，并且通过Western分析检测到。从假单胞菌OF4中删除atpZ，atpI或atpZI导致需要在pH 7.5下生长的Mg2 +浓度大大增加。 atpZ，atpI或atpZI均可补充鼠伤寒沙门氏菌（MM281）的三重突变体的相似表型，后者缺乏Mg2 +吸收。 atpZ和atpI分别和一起增加了Ca2 +和Na +流出缺陷的大肠杆菌突变体的囊泡对Mg2 +敏感的45Ca2 +的吸收。我们假设AtpZ和AtpI是Mg2 +转运蛋白，Ca2 +转运蛋白或通道蛋白，属于均聚物，也可能属于杂聚物。当该酶在水解方向上起作用时，此类蛋白质可以提供ATP合酶所需的Mg2 +，并且还支持电荷补偿。例如在细胞质pH调节期间。

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