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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets.
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A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets.

机译：仅在减数分裂或减数分裂后生精细胞中表达的大量基因提供了无数的避孕靶标。

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Understanding mammalian spermatozoan development and the events surrounding fertilization has grown slowly, in part because of uncertainty about the number and identity of the cellular components involved. Determination of those transcripts expressed specifically by germ cells should provide an inclusive list of probable critical proteins. Here, total mouse testis transcript profiles were trimmed of transcripts found in cultures enriched in Sertoli or interstitial cells to yield a germ cell-enriched transcript profile. Monitoring of changes of this profile in the developing testis identified 1,652 genes whose transcript abundance increased markedly coincident with the onset of meiosis. Remarkably, 351 of these genes ( approximately 20%) appear to be expressed only in the male germline. Germ cell-specific transcripts are much less common earlier in testis development. Further analysis of the UniGene EST database coupled with quantitative PCR indicates that approximately 4% of the mouse genome is dedicated to expression in postmeiotic male germ cells. Most or many of the protein products of these transcripts are probably retained in mature spermatozoa. Targeted disruption of 19 of these genes has indicated that a majority have roles critical for normal fertility. Thus, we find an astonishing number of genes expressed specifically by male germ cells late in development. This extensive group provides a plethora of potential targets for germ cell-directed contraception and a staggering number of candidate proteins that could be critical for fertilization.
机译：对哺乳动物精子动物的发育和受精事件的了解已缓慢发展，部分原因是所涉及的细胞成分的数量和特性尚不确定。确定由生殖细胞特异性表达的那些转录本应提供可能的关键蛋白质的完整清单。在这里，将小鼠睾丸的全部转录本图谱修剪成富含Sertoli或间质细胞的培养物中发现的转录本，以产生生殖细胞富集的转录本图谱。在发育中的睾丸中监测此轮廓的变化可鉴定出1,652个基因，其转录丰度与减数分裂的发生显着重合。值得注意的是，这些基因中的351个（约20％）似乎仅在雄性种系中表达。生殖细胞特异的转录本在睾丸发育早期较不常见。 UniGene EST数据库与定量PCR的进一步分析表明，大约4％的小鼠基因组致力于减数分裂后雄性生殖细胞中的表达。这些转录本的大部分或许多蛋白质产物可能保留在成熟的精子中。这些基因中的19个被有针对性地破坏表明，大多数基因对正常生育具有关键作用。因此，我们发现了雄性生殖细胞在发育后期专门表达的惊人数量的基因。这一广泛的群体为生殖细胞定向避孕提供了许多潜在的靶标，并且提供了数量惊人的候选蛋白，这些蛋白对于受精至关重要。

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