A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA-protein interactions

P. C. Joost Haasnoot; John F. Bol; Rene C. L. Olsthoorn

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A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA-protein interactions

机译：一种植物病毒复制系统，可检测RNA-蛋白质相互作用中RNA假三环基序的形成

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A pseudotriloop is formed by transloop base pairing between the first (5′) and the fifth nucleotide in a hexanucleotide RNA loop ("hexaloop") to subtend a triloop of nucleotides 2-4. This structure has been found in hairpins involved in the regulation of iron metabolism in mammalian cells and in transcription of plant virus subgenomic RNA. Several hexaloop hairpins, including HIV-trans-activation-responsive element and hepatitis B virus ε, potentially adopt a pseudotriloop conformation. Here we show that an RNA plant virus whose replication depends on a conventional triloop hairpin can be used to verify the existence of pseudotriloop structures in vivo. Our data suggest that the pseudotriloop may represent a common motif in RNA-protein recognition.

机译：通过在六核苷酸RNA环（“ hexaloop”）中的第一个（5'）和第五个核苷酸之间进行反式环碱基配对以形成核苷酸2-4的三环而形成伪三环。在发夹中发现了这种结构，所述发夹涉及哺乳动物细胞中铁代谢的调节以及植物病毒亚基因组RNA的转录。几个六环发夹，包括HIV反式激活应答元件和乙型肝炎病毒ε，可能采用假三环构象。在这里，我们显示了其复制依赖于常规三环发夹的RNA植物病毒可用于验证体内假三环结构的存在。我们的数据表明伪三环可能代表RNA蛋白质识别中的常见主题。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2003年第22期|p.12596-12600|共5页
作者
P. C. Joost Haasnoot; John F. Bol; Rene C. L. Olsthoorn;
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作者单位

Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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6. A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA–protein interactions [O] . P. C. Joost Haasnoot, John F. Bol, René C. L. Olsthoorn 2003

机译：一种植物病毒复制系统可检测RNA-蛋白质相互作用中RNA假三环基序的形成
7. A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA–protein interactions [O] . Haasnoot, P. C. Joost, Bol, John F., Olsthoorn, René C. L. 2003

机译：一种植物病毒复制系统，可检测RNA-蛋白质相互作用中RNA假三环基序的形成

A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA-protein interactions

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