Toward development of a screen to identify randomly encoded, foldable sequences

Yoshihisa Hagihara; Peter S. Kim

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Toward development of a screen to identify randomly encoded, foldable sequences

机译：致力于开发屏幕以识别随机编码的可折叠序列

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The ability to identify sequences in a randomly encoded polypep- tide library that are capable of acquiring unique and stably folded structures would be valuable in the examination of protein-folding issues. The quality control system of the yeast secretory pathway prevents the release of incompletely folded polypeptides. Earlier work has shown that this feature can be used in a screen to identify mutations that increase the stability of a protein. We sought to extend this strategy for use with random sequence libraries by combining a quality-control system-based screen with generic tag-based immunodetection that can be applied to any sequence.

机译：在随机编码的多肽文库中鉴定能够获得独特且稳定折叠的结构的序列的能力在检查蛋白质折叠问题中将是有价值的。酵母分泌途径的质量控制系统防止释放不完全折叠的多肽。早期的工作表明，可以在屏幕上使用此功能来识别可增加蛋白质稳定性的突变。我们试图通过将基于质量控制系统的筛选与可应用于任何序列的基于通用标签的免疫检测相结合，来扩展该策略以用于随机序列文库。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2002年第10期|p.6619-6624|共6页
作者
Yoshihisa Hagihara; Peter S. Kim;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
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1. Toward development of a screen to identify randomly encoded, foldable sequences [J] . Yoshihisa Hagihara, Peter S. Kim Proceedings of the National Academy of Sciences of the United States of America . 2002,第10期

机译：致力于开发屏幕以识别随机编码的可折叠序列
2. Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDnas identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries. [J] . Okazaki N, Kikuno R, Ohara R, DNA research: an international journal for rapid publication of reports on genes and genomes . 2004,第2期

机译：小鼠FLJ基因同源物编码序列的预测：通过筛选从大小分级文库中随机取样的cDNA克隆的末端序列，鉴定出110个小鼠FLJ同源cDnas的完整核苷酸序列。
3. Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated librari [J] . Okazaki N, F Kikuno R, Ohara R, DNA research: an international journal for rapid publication of reports on genes and genomes . 2004,第3期

机译：KIAA基因的小鼠同源物编码序列的预测：IV。通过筛选从大小分级文库中随机取样的cDNA克隆的末端序列来鉴定500个小鼠KIAA同源cDNA的完整核苷酸序列
4. Development of Combinatorial Peptide Screening to Identify Novel Nonfouling Sequences [C] . Ann K. Nowinski, Andrew J. Keefe, Andrew D. White, Society for Biomaterials annual meeting and exposition . 2014

机译：鉴定新的非污染序列的组合肽筛选的发展。
5. Identifying functional lox sequences: A genomic search and randomized libraries. [D] . Sheren, Jamie Elizabeth. 2007

机译：识别功能性lox序列：基因组搜索和随机文库。
6. Toward development of a screen to identify randomly encoded foldable sequences [O] . Yoshihisa Hagihara, Peter S. Kim 2002

机译：致力于开发屏幕以识别随机编码的可折叠序列
7. Toward development of a screen to identify randomly encoded, foldable sequences [O] . Hagihara, Yoshihisa, Kim, Peter S. 2002

机译：致力于开发屏幕以识别随机编码的可折叠序列
8. Genetic engineering with a gene encoding a soybean storage protein to identify DNA sequences to control its expression: Annual report, 1993 [R] . Beachy, R N 1993

机译：用编码大豆贮藏蛋白的基因进行基因工程鉴定DNa序列以控制其表达：年度报告，1993年

Toward development of a screen to identify randomly encoded, foldable sequences

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