AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization

Cristina Rada; John M. Jarvis; Cesar Milstein

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AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization

机译：AID-GFP嵌合蛋白可增加Ig基因的超突变，而无核定位证据

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Somatic hypermutation generates variants of antibody genes and underpins the affinity maturation of antibodies. It is restricted to the V-gene segments, and although it decays exponentially toward the 3'end, it includes recognizable hot spots. Although the detailed mechanism of hypermutation remains elusive, the process may take place in two separate stages, preferentially targeting G/Cs in the first and A/Ts in the second stage. It seems that MSH2 is involved in the second stage, and that activation induced deami- nase (AID) is implicated in the control of hypermutation. The constitutively hypermutating cell line Ramos expresses AID, and we have prepared transfectants that express a chimeric AID-green fluorescent protein.

机译：体细胞超突变产生抗体基因的变体，并支持抗体的亲和力成熟。它仅限于V基因片段，尽管它向3'端呈指数衰减，但它包含可识别的热点。尽管高变的详细机制仍然难以捉摸，但是该过程可能会在两个单独的阶段进行，优先针对第一阶段的G / C和第二阶段的A / T。似乎MSH2参与了第二阶段，激活诱导的脱氨酶（AID）参与了超突变的控制。组成性超突变细胞系Ramos表达AID，我们已经制备了表达嵌合AID绿色荧光蛋白的转染子。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2002年第10期|p.7003-7008|共6页
作者
Cristina Rada; John M. Jarvis; Cesar Milstein;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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机译：AID-GFP嵌合蛋白可增加lg基因的超突变，而无核定位证据
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机译：使用毛细管电泳分析单细胞水平上的核制剂和融合蛋白的核定位的研究。
6. AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization [O] . Cristina Rada, John M. Jarvis, César Milstein 2002

机译：AID-GFP嵌合蛋白可增加Ig基因的超突变而无核定位证据
7. AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization [O] . Rada, Cristina, Jarvis, John M., Milstein, César 2002

机译：AID-GFP嵌合蛋白可增加Ig基因的超突变，而无核定位证据
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机译：具有神经可见蛋白样钙结合域的嵌合植物钙/钙调蛋白依赖性蛋白激酶基因

AID-GFP chimeric protein increases hypermutation of Ig genes with no evidence of nuclear localization

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