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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene hiosynthesis in Phycomyces
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A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene hiosynthesis in Phycomyces

机译：番茄红素环化酶，八氢番茄红素合酶和芽孢杆菌中胡萝卜素的合成调控的单一基因

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摘要

Previous complementation and mapping of mutations that change the usual yellow color of the Zygomycete Phycomyces blakesleea- nus to white or red led to the definition of two structural genes for carotene biosynthesis. We have cloned one of these genes, carRA, by taking advantage of its close linkage to the other, carB, respon- sible for phytoene dehydrogenase. The sequences of the wild type and six mutants have been established, compared with sequences in other organisms, and correlated with the mutant phenotypes. The carRA and carB coding sequences are separated by 1,381 untranslated nucleotides and are divergently transcribed. Gene carRA contains separate domains for two enzymes, lycopene cy- clase and phytoene synthase, and regulates the overall activity of the pathway and its response to physical and chemical stimuli from the environment. The lycopene cyclase domain of carRA derived from a duplication of a gene from a common ancestor of fungi and Brevibacterium linens; the phytoene synthase domain is similar to the Phytoene and squalene synthases of many organisms: but the regulatory functions appear to be specific to Phycomyces.
机译：以前的互补和突变定位（将Zygomycete Phycomyces blakesleeaus的通常的黄色变成白色或红色）导致了胡萝卜素生物合成的两个结构基因的定义。我们利用与phytoene脱氢酶负责的另一个carB的紧密连接，克隆了其中一个基因carRA。与其他生物中的序列相比，已建立了野生型和六个突变体的序列，并与突变体表型相关。 carRA和carB编码序列被1,381个未翻译的核苷酸隔开，并被不同地转录。基因carRA包含番茄红素环化酶和植物六烯合酶这两种酶的独立结构域，并调节该途径的整体活性及其对环境物理和化学刺激的反应。 carRA的番茄红素环化酶结构域源自真菌​​和短杆菌属的共同祖先的基因重复；八氢番茄红素合酶结构域与许多生物的八氢番茄红素和角鲨烯合酶相似：但是调节功能似乎是针对藻类的。

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