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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I
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Suppression of chromosome segregation defects of Escherichia coli muk mutants by mutations in topoisomerase I

机译：拓扑异构酶I突变抑制大肠埃希氏菌muk突变体的染色体分离缺陷

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摘要

Escherichia coli muk mutants are temperature-sensitive and pro- duce anucleate cells. A spontaneously occurring mutation was found in a AmukB::kan mutant strain that suppressed the temper- ature-sensitive phenotype and mapped in or near topA. the gene that encodes topoisomerase l. Previously characterized topA mu- tatans, topA10 and topA66, were found to be general suppressors 0f muk mutants: they suppressed temperature sensitivity and anucleate cell production of cells containing null or point muta- tions in mukB and null mutations in mukE or mukF. The suppression torrelated with excess negative supercoiling by DNA gyrase. and the gyrase inhibitor, coumermycin. reversed it. Defects in topA aIIow 99/100 of cell division events in muk null mutants to proceed without chromosome loss or loss of cell viability. This observation imposes important limitations on models for Muk activity and is consistent with a role for MukBEF in chromosome folding and DNA condensation.
机译：大肠杆菌muk突变体对温度敏感，并产生无核细胞。在AmukB :: kan突变株中发现了自发突变，该突变株可抑制温度敏感性表型并定位在topA或topA附近。编码拓扑异构酶l的基因。发现先前表征的topA突变体topA10和topA66是通用抑制物0f muk突变体：它们抑制了温度敏感性和mukB中含有无效突变或点突变以及mukE或mukF中无效突变的细胞的无核细胞生成。 DNA促旋酶与过度负超螺旋的抑制作用有关。以及回旋酶抑制剂coumermycin。扭转它。 muk null突变体中topA的细胞分裂事件中的99/100缺陷继续进行，而没有染色体损失或细胞活力丧失。该观察对Muk活性的模型强加了重要限制，并且与MukBEF在染色体折叠和DNA浓缩中的作用一致。

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