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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Involvement of multiple intracellular release channels in calcium sparks of skeletal muscle
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Involvement of multiple intracellular release channels in calcium sparks of skeletal muscle

机译：多个细胞内释放通道参与骨骼肌钙火花的产生

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摘要

In many types of muscle. intracellular Ca~2+ release for contraction consists of brief Ca~2+ sparks. Whether these result from the opening of one or many channels in the sarcoplasmic reticulum is not known. Examining massive numbers of sparks from frog skeletal muscle and evaluating their Ca~2+ release current, we provide evidence that they are generated by multiple channels. A mode is demonstrated in the distribution of spark rise times in the presence of the channel activator caffeine. This finding contradicts expectations for single channels evolving reversibly, but not for channels in a group. which collectively could give rise to a stereo- typed spark. The release channel agonists imperatoxin A, ryano- dine. and bastadin 10 elicit fluorescence events that start with a spark. then decay to steady levels roughly proportional to the unitary conductances of 35/100, 50/199, and 100/100 that the agonists, respectively. promote in bilayer experiments. This correspondence indicates that the steady phase is produced by one open channel. Calculated Ca2+ release current decays 10- to 20-fold from spark to steady phase, which requires that six or more channels be open during the spark.
机译：在许多类型的肌肉中。细胞内Ca〜2 +的释放释放是由短暂的Ca〜2 +火花引起的。这些是否由肌浆网中一个或多个通道的开放所致尚不清楚。检查青蛙骨骼肌产生的大量火花并评估其Ca〜2 +释放电流，我们提供了证据，表明它们是由多个通道产生的。在通道活化剂咖啡因的存在下，在火花上升时间的分布中证明了一种模式。这一发现与对单个渠道可逆发展的期望相抵触，但与对一组渠道的期望相矛盾。共同会引起定型火花。释放通道激动剂imperatoxin A，ryanodine。 Bastadin 10引发以火花开始的荧光事件。然后衰减到稳定水平，分别与激动剂的单位电导分别成比例的35 / 100、50 / 199和100/100。促进双层实验。该对应关系表明稳定相是由一个明渠产生的。计算得出的Ca2 +释放电流从火花到稳定相会衰减10到20倍，这需要在火花过程中打开六个或更多通道。

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