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首页> 外文会议>Society of Photo-Optical Instrumentation Engineers Conference on Biomarkers and Biological Spectral Imaging >Dimensions of calcium release domains in frog skeletal muscle fibers
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Dimensions of calcium release domains in frog skeletal muscle fibers

机译：青蛙骨骼肌纤维中钙释放域的尺寸

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The spatiotemporal properties of the Ca~(2+) release process in skeletal muscle fibers were determined using an improved confocal spot detection system. Muscle fibers were loaded with the low affinity fluorescent Ca~(2+) indicator OGB-5N and localized action potential-induced fluorescence signals were recorded from consecutive locations separated by 200 nm within a single sarcomere. Three-dimensional reconstructions of the Ca~(2+) transients illustrate the existence of domains of increased fluorescence around Ca~(2+) release sites in the neighborhood of the T-tubules. We estimated the dimensions of these domains by drawing isochronal curves (ΔF/F vs. spot position) and fitting Gaussian profiles to them. It was found that the earliest detectable full-width-at-half-maximum of these profiles was 0.77 ± 0.25 μm and increased rapidly with time to 1.4 ± 0.2 μm at their peak (18 °C). A brief, but statistically significant, delay of 0.8 ± 0.42 ms was observed between the onset of the fluorescence transients at the Z- and M-lines. Our results are compatible with the possibility that, in response to AP stimulation, Ca~(2+) is not released exclusively from the junctional region of the sarcoplasmic reticulum, but from a broader expanse of the triadic region.
机译：钙〜（2+）释放过程中骨骼肌纤维中的时空性能用改进的共聚焦点探测系统确定的。肌纤维装载有低亲和力的荧光的Ca〜（2+）指示器OGB-5N和局部的动作电位引起的荧光信号从连续位置记录在单独的肌节内由200nm的分离。钙〜的三维重建（2+）瞬变说明增加周围的Ca〜（2+）在T管的附近释放位点的荧光的结构域的存在。我们通过绘制等时曲线（ΔF/ F与光点位置），并将它们嵌合高斯分布曲线估算出这些结构域的尺寸。据发现，最早检测的全宽在半最大的这些轮廓为0.77±0.25微米，并在它们的峰值（18℃）随时间迅速增加至1.4±0.2微米。的0.8±0.42毫秒一个短暂的，但在统计学上显著，延迟在Z-和M线的荧光瞬变的发作之间观察到了。我们的结果与响应于AP刺激，钙〜（2+）不完全是从肌质网的交界区域释放的可能性兼容，但是从三元区域的更宽的宽阔。

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