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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Leadinq and lagging strand DNA synthesis by a reconstituted herpes simplex virus type 1 replisome
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Leadinq and lagging strand DNA synthesis by a reconstituted herpes simplex virus type 1 replisome

机译：重组单纯疱疹病毒1型复制体的Leadinq和落后链DNA合成

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摘要

The synthesis of double-stranded DNA by a rolling circle mecha- nism was reconstituted in vitro with a replisome consisting of the DNA polymerase-UL42 complex and the heterotrimeric helicase- primase encoded by herpes simplex virus type 1. Okazaki frag- ments 3 kilobases in length and leading strands that may exceed 10 kilobases are produced. Lagging strand synthesis is stimulated by ribonucleoside triphosphates. DNA replication appears to be processive because it resists competition with an excess of (dT)150/ (dA)_20. The single-strand DNA binding protein ICP8 is not required, and high concentrations of lCP8 can, in fact, inhibit lagging strand synthesis. The inhibition can, however, be overcome by the addi- tion of an excess of the UL8 component of the helicase-primase. Rolling circle replication by the herpesvirus and bacteriophage T7 replisomes appears to proceed by a similar mechanism.
机译：通过滚环机制合成的双链DNA在体外用由DNA聚合酶-UL42复合体和1型单纯疱疹病毒编码的异三聚解旋酶-primase组成的复制体重建。Okazaki片段在3 kb内产生的长度和前导股可能超过10公里。滞后链的合成受到核糖核苷三磷酸的刺激。 DNA复制似乎具有持续性，因为它可以抵抗（dT）150 /（dA）_20过量的竞争。不需要单链DNA结合蛋白ICP8，实际上，高浓度的LCP8可以抑制滞后链的合成。但是，可以通过添加过量的解旋酶-primase的UL8组分来克服这种抑制作用。疱疹病毒和噬菌体T7复制体的滚环复制似乎是通过类似的机制进行的。

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