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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Wnt/Shh interactions regulate ectodermal boundary formation during mammalian tooth development
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Wnt/Shh interactions regulate ectodermal boundary formation during mammalian tooth development

机译：Wnt / Shh相互作用调节哺乳动物牙齿发育过程中外胚层边界的形成

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摘要

Interactions between the Wnt (wingless) and hedgehog signaling pathways were first described as playing a role in establishing boundaries between ectodermal cells in Drosophila segmentation. During the initiation of mammalian tooth development, bound- aries that distinguish oral from dental ectoderm must be formed to correctly position the sites of tooth formation. We describe a reciprocal relationship between the expression of Wnt-7b in pre- sumptive oral ectoderm and Shh in presumptive dental ectoderm in mouse embryos that mark boundaries between these cells with different developmental fates. By using a murine retrovirus to ectopically express Wnt-7b in presumptive dental ectoderm in mandibular arch explants, we show that Shh expression in the ectoderm and Ptc expression in the underlying ectomesenchyme are down--regulated, and tooth development is subsequently ar- rested. This suggests that Wnt-7b acts to repress Shh expression in oral ectoderm. thus maintaining the boundaries between oral and dental ectodermal cells. Implantation of beads soaked in Shh protein into Wnt-7b-infected explants resulted in complete rescue of tooth development. confirming that the repressive action of Wnt-7b specifically affects Shh signaling.
机译：Wnt（无翅）和刺猬信号通路之间的相互作用首先被描述为在果蝇分割中建立外胚层细胞之间的边界中发挥作用。在哺乳动物牙齿发育的初始阶段，必须形成区分口腔和牙齿外胚层的边界，以正确定位牙齿的形成位置。我们描述了假定的口腔外胚层中Wnt-7b的表达与假定的牙齿外胚层中Shh在小鼠胚胎中的表达之间的相互关系，这些标志着这些细胞之间具有不同发育命运。通过使用鼠逆转录病毒在下颌牙弓外植体的假定牙齿外胚层中异位表达Wnt-7b，我们显示出外胚层中的Shh表达和潜在的外间质中的Ptc表达下调，并随后阻止了牙齿发育。这表明Wnt-7b可抑制口腔外胚层的Shh表达。从而维持口腔和牙齿外胚层细胞之间的界限。将浸泡在Shh蛋白中的珠子植入感染Wnt-7b的外植体中，可以完全拯救牙齿的发育。证实了Wnt-7b的抑制作用特别影响Shh信号传导。

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