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Application of cellulase-polyamidoamine dendrimer-modified silica for microwave-assisted chitosan enzymolysis

机译：纤维素酶-聚酰胺酰胺树状聚合物改性的二氧化硅在微波辅助壳聚糖酶解中的应用

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Polyamidoamine dendrimer (PAMAM) is one of a number of dendritic polymers with a precise molecular structure, high geometric symmetry, and a large number of terminal groups. In this study, PAMAM was grafted onto the surface of silica by microwave irradiation and characterized by Fourier transform infrared spectroscopy and elemental analysis. A novel immobilized cellulase was developed based on enzyme immobilization onto the prepared PAMAM-grafted silica and applied in microwave-assisted chitosan enzymolysis. The results show that the efficiency of cellulase immobilization increased with increasing generations of PAMAM. A high enzymatic hydrolysis efficiency was obtained for a 7 mg ml~(-1) chitosan solution at pH 6.2 and 50 C with 40 W microwave-assisted enzymolysis (20 min) compared with a conventional enzymolysis protocol (3 h). The experimental results indicate that this rapid and efficient enzymolysis method combines the advantages of both PAMAM and microwave-assisted technology, which can be adapted to high-throughput enzyme assay in biochemical and clinical research.
机译：聚酰胺基胺树枝状聚合物（PAMAM）是许多具有精确分子结构，高几何对称性和大量末端基团的树枝状聚合物之一。在这项研究中，通过微波辐射将PAMAM接枝到二氧化硅表面，并通过傅里叶变换红外光谱和元素分析对其进行表征。基于将酶固定在制备的PAMAM接枝的二氧化硅上的酶，开发了一种新型的固定纤维素酶，并将其应用于微波辅助的壳聚糖酶解中。结果表明，纤维素酶固定效率随PAMAM世代的增加而增加。与传统的酶解方案（3 h）相比，在40 W微波辅助的酶解（20分钟）下，在pH 6.2和50°C的条件下，对于7 mg ml〜（-1）的壳聚糖溶液，酶解效率较高。实验结果表明，这种快速有效的酶解方法结合了PAMAM和微波辅助技术的优点，可适用于生化和临床研究中的高通量酶分析。

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