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首页> 外文期刊>Process Biochemistry >Enhanced synthesis of medium-chain-length poly(3-hydroxyalkanoates) by inactivating the tricarboxylate transport system of Pseudomonas putida KT2440 and process development using waste vegetable oil
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Enhanced synthesis of medium-chain-length poly(3-hydroxyalkanoates) by inactivating the tricarboxylate transport system of Pseudomonas putida KT2440 and process development using waste vegetable oil

机译：通过灭活恶臭假单胞菌KT2440的三羧酸盐运输系统来增强中链聚（3-羟基链烷酸酯）的合成和使用废植物油的工艺开发

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The use of waste materials as feedstock for biosynthesis of valuable compounds has been an intensive area of research aiming at diminishing the consumption of non-renewable materials. In this study, P. putida KT2440 was employed as a cell factory for the bioconversion of waste vegetable oil into medium-chain-length Polyhydroxyalkanoates. In the presence of the waste oil this environmental strain is capable of secreting enzymes with lipase activities that enhance the bioavailability of this hydrophobic carbon substrate. It was also found that the oxygen transfer coefficient is directly correlated with high PHA levels in KT2440 cells when metabolizing the waste frying oil. By knocking out the tctA gene, encoding for an enzyme of the tripartite carboxylate transport system, an enhanced intracellular level of me-PHA was found in the engineered strain when grown on fatty acids. Batch bioreactors showed that the KT2440 strain produced 1.01 (g.L-1) of PHA whereas the engineered Delta tctA P. putida strain synthesized L91 (g.L-1) after 72 h cultivation on 20 (g.L-1) of waste oil, resulting in a nearly 2-fold increment in the PHA volumetric productivity. Taken together, this work contributes to accelerate the pace of development for efficient bioconversion of waste vegetable oils into sustainable biopolymers.
机译：为了减少不可再生材料的消耗，使用废料作为生物合成有价值化合物的原料已经成为研究的重点领域。在这项研究中，恶臭假单胞菌KT2440被用作细胞工厂，将废植物油生物转化为中链长度的聚羟基链烷酸酯。在废油存在下，该环境菌株能够分泌具有脂肪酶活性的酶，从而增强该疏水性碳底物的生物利用度。还发现，在代谢废煎炸油时，氧传递系数与KT2440细胞中的高PHA水平直接相关。通过敲除编码三方羧酸盐转运系统酶的tctA基因，当在脂肪酸上生长时，在工程菌株中发现了增强的细胞内me-PHA水平。分批生物反应器显示，KT2440菌株产生1.01（gL-1）的PHA，而经过改造的Delta tctA恶臭假单胞菌菌株在20（gL-1）的废油上培养72小时后合成了L91（gL-1），从而产生了PHA的容积生产率几乎提高了2倍。总之，这项工作有助于加快将废植物油有效地生物转化为可持续生物聚合物的开发步伐。

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