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Improving the production of fungal exopolysaccharides with application of repeated batch fermentation technology coupling with foam separation in the presence of surfactant

机译:通过在表面活性剂存在下应用重复分批发酵技术偶联的重复批量发酵技术耦合及其在泡沫分离中提高真菌脱寡糖的生产

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摘要

Biotechnology is designed to provide enhanced better services to human life. The current manuscript focuses on the development of exopolysaccharides (EPSs) produced by Cordyceps militaris in order to solve the problems of low productivity and high cost in downstream separation. The experiment of repeated batch fermentation coupling with foam separation in the presence of a surfactant was designed to achieve this goal. The EPSs production reached 1.313 g/L by adding 0.1 % (v/v) of tween 80 on the 4th day. Repeated batch fermentation coupling with foam separation was performed for five batches in 13 days. During the coupling process, the enrichment ratio and recovery percentage of EPSs were maintained at 2.4 and 60 %, respectively. Meanwhile, at recycling processes, the mycelium from the foamate of batch II displayed EPSs production of 1.567 g/L, which was higher than the control group (1.024 g/L). The study demonstrated that this technology would be promising for improving the EPSs production.
机译:生物技术旨在为人类生命提供增强的更好服务。目前的手稿侧重于冬虫夏草生产中产生的外偶糖(EPS),以解决低于生产率和下游分离成本的问题。设计了在表面活性剂存在下与泡沫分离的重复分批发酵偶联的实验才能实现这一目标。通过在第4天添加0.1%(v / v),EPS生产达到1.313克/升。在13天内进行五批次进行与泡沫分离的重复分批发酵偶联。在偶联过程中,EPS的富集率和恢复百分比分别保持在2.4和60%。同时,在回收过程中,菌丝体来自批量II的泡沫塑料展示的EPSS产量为1.567克/升,高于对照组(1.024克/升)。该研究表明,这项技术将有助于改善EPS生产。

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