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Immunocytochemistry of pectins in shoot apical meristems: consequences for intercellular adhesion

机译:果胶在茎尖分生组织中的免疫细胞化学:细胞间粘附的后果

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摘要

The nature of pectins (acidic, methyl-, or acetyl-esterified) in the shoot meristem of Sinapis alba was assessed by immunocytochemistry with the 2F4 monoclonal antibody in light and electron microscopy. This antibody is specific for “egg-boxes” – the polygalacturonic acid conformation induced by calcium as described in Liners et al. (Plant Physiol. 99: 1099–1104, 1992). Hardly any acidic pectin was detected in meristem walls; the pectins were largely methyl-esterified and esterified by acetyl groups and/or other esters. After in situ chemical or enzymatic de-esterification, labeling was distributed over the primary wall and the middle lamella of meristematic cells. Acidic pectin and Ca2+-cross-linked homogalacturonans were absent from the pit fields, where plasmodesmata traverse the middle lamella. The type and distribution of pectins are discussed in relation to cellular adhesion between active meristem cells.
机译:通过光和电子显微镜用2F4单克隆抗体通过免疫细胞化学评估了芥末茎分生组织中果胶(酸性,甲基或乙酰基酯化)的果胶性质。该抗体对“蛋盒”具有特异性,如Liners等人所述,由钙诱导的聚半乳糖醛酸构象。 (Plant Physiol。99:1099-1104,1992)。在分生组织壁上几乎未检测到酸性果胶;果胶大部分被甲基酯化并被乙酰基和/或其他酯酯化。原位化学或酶解酯化后,标记分布在分生细胞的初生壁和中层。坑田不存在酸性果胶和Ca 2+交联的同型半乳糖醛酸,其中浆线虫横穿中层。果胶的类型和分布与活性分生组织细胞之间的细胞粘附有关。

著录项

  • 来源
    《Protoplasma》 |2005年第2期|15-22|共8页
  • 作者单位

    Unité de Recherches en Biologie Cellulaire Végétale Département de Biologie Facultés Universitaires Notre-Dame de la Paix;

    Laboratoire de Physiologie Végétale Département des Sciences de la Vie Université de Liége;

    Unité de Recherches en Biologie Cellulaire Végétale Département de Biologie Facultés Universitaires Notre-Dame de la Paix;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Key words: Adhesion; Calcium gel; “Egg-box” conformation; Sinapis alba; Meristem; Pectin.;

    机译:关键字:附着力;钙凝胶;“鸡蛋盒”构象;芥菜分生组织;果胶。;

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