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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Immunocytochemistry of pectins in shoot apical meristems: consequences for intercellular adhesion
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Immunocytochemistry of pectins in shoot apical meristems: consequences for intercellular adhesion

机译：果胶在茎尖分生组织中的免疫细胞化学：细胞间粘附的后果

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摘要

The nature of pectins ( acidic, methyl-, or acetyl-esterified) in the shoot meristem of Sinapis alba was assessed by immunocytochemistry with the 2F4 monoclonal antibody in light and electron microscopy. This antibody is specific for "egg-boxes'' - the polygalacturonic acid conformation induced by calcium as described in Liners et al. ( Plant Physiol. 99: 1099 - 1104, 1992). Hardly any acidic pectin was detected in meristem walls; the pectins were largely methyl- esterified and esterified by acetyl groups and/or other esters. After in situ chemical or enzymatic de-esterification, labeling was distributed over the primary wall and the middle lamella of meristematic cells. Acidic pectin and Ca2+-cross-linked homo-galacturonans were absent from the pit fields, where plasmodesmata traverse the middle lamella. The type and distribution of pectins are discussed in relation to cellular adhesion between active meristem cells.
机译：通过光和电子显微镜用2F4单克隆抗体进行免疫细胞化学评估了芥末茎分生组织中果胶（酸性，甲基或乙酰基酯化）的果胶性质。该抗体对“蛋盒”具有特异性，如Liners等人（植物生理学99：1099-1104，1992）所述，由钙诱导的聚半乳糖醛酸构象。在分生组织壁中几乎未检测到任何酸性果胶;果胶大部分被甲基酯化并被乙酰基和/或其他酯类酯化，原位化学或酶促去酯化后，标记物分布在分生细胞的初生壁和中层，酸性果胶与Ca2 +交联坑内不存在同型半乳糖醛酸，而浆膜横穿中层，果胶的类型和分布与活跃的分生组织之间的细胞粘附有关。

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