Hemoglobin Tokyo II[α89 (FG1)His-Asn]: A new Hemoglobin Variant Characterized by Endoproteinase Asp-N Digestion and Collision-Induced Dissociation

Tomokazu Nakano; Yoshinao Wada

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Hemoglobin Tokyo II[α89 (FG1)His-Asn]: A new Hemoglobin Variant Characterized by Endoproteinase Asp-N Digestion and Collision-Induced Dissociation

机译：东京血红蛋白II [α89（FG1）His-Asn]：以内蛋白酶Asp-N消化和碰撞诱导解离为特征的新血红蛋白变异体

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The presence of a hemoglobin mutant was suggested in a male subject who showed an apparently false level of glycated hemoglobin based on ion-exchange chromatography. Electrospray ionization mass spectrometry detected a mutant molecule with a mass of 15, 104units, 23 units less than normal α-globin. The mutation was identified in a large tryptic peptide, αT9, of residues 62-90. Endoproteinase Asp-N digestion of carboxymethyl- ated α-globin cleved the region into peptides of ca. 1,000 Da, and localized the mutation to the peptide corresponding to residues 85-94.

机译：男性受试者中提示存在血红蛋白突变体，基于离子交换色谱法显示糖化血红蛋白水平明显错误。电喷雾电离质谱法检测到质量为15个104单位的突变分子，比正常的α-珠蛋白少23个单位。在大的胰蛋白酶肽αT9的残基62-90中鉴定出该突变。羧甲基化的α-球蛋白的内切蛋白酶Asp-N消化将区域切割成大约ca的肽。 1000Da，并将突变定位于对应于残基85-94的肽。

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《Journal of the Mass Spectrometry Society of Japan》 |2000年第5期|p.341-345|共5页
作者
Tomokazu Nakano; Yoshinao Wada;
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正文语种 jpn
中图分类分析化学;一般工业技术;
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1. Hemoglobin Tokyo II[α89 (FG1)His-Asn]: A new Hemoglobin Variant Characterized by Endoproteinase Asp-N Digestion and Collision-Induced Dissociation [J] . Tomokazu Nakano, Yoshinao Wada Journal of the Mass Spectrometry Society of Japan . 2000,第5期

机译：东京血红蛋白II [α89（FG1）His-Asn]：以内蛋白酶Asp-N消化和碰撞诱导解离为特征的新血红蛋白变异体
2. Hb Neuilly-sur-Marne, a new human hemoglobin variant with Ser-Asp-Leu inserted between α86(F7) Leu and α87(F8) His: characterization by high-energy collision-induced dissociation liquid secondary ion mass spectrometry and low energy collision-induc [J] . Danielle Prome, Jean-Claude Prome, Henri Wajcman, European journal of mass spectrometry . 2000,第2期

机译：Hb Neuilly-sur-Marne，一种新的人类血红蛋白变异体，在α86（F7）Leu和α87（F8）His之间插入了Ser-Asp-Leu：通过高能碰撞诱导的解离液体二次离子质谱和低能碰撞进行表征-诱导
3. Mass spectrometry and DNA sequencing are complementary techniques for characterizing hemoglobin variants: the example of hemoglobin J-Oxford | Haematologica [J] . D Caruso, M Crestani, L Da Riva, Haematologica . 2004,第5期

机译：质谱和DNA测序是表征血红蛋白变异体的补充技术：血红蛋白J-Oxford的实例|血液学
4. Application of multiple protease digestion with SPS de novo approach to the characterization of hemoglobin variants [C] . Jane Y. Yang, Adrian Guthals, Jesse Meyer, ASMS Conference on Mass Spectrometry and Allied Topics . 2014

机译：多种蛋白酶消化用SPS DE Novo方法在血红蛋白变体表征中的应用
5. Development and validation of an oxygen dissociation assay a screening platform for discovering and characterizing hemoglobin–oxygen affinity modifiers [O] . Mira P Patel, Vincent Siu, Abel Silva-Garcia, 2018

机译：氧离解测定法的开发和验证一种用于发现和表征血红蛋白-氧亲和性修饰剂的筛选平台
6. Hemoglobin Tokyo II .ALPHA.89(FG1) His.RAR.Asn: A New Hemoglobin Bariant Characterized by Endoproteinase Asp-N Digestion and Collision-Induced Dissociation. [O] . Tomokazu NAKANO, Yoshinao WADA 2000

机译：血红蛋白Tokyo II .Alpha.89（FG1）His.rar.asn：一种新的血红蛋白横贯体，其特征在于内蛋白酶ASP-N消化和碰撞诱导的解离。
7. Hemoglobin variants as models for investigation of dissociation of intact polypeptide chains by ESI tandem mass spectrometry. [R] . K. J. Light J. A. Loo C. G. Edmonds R. D. Smith 1991

机译：血红蛋白变体作为用于通过EsI串联质谱法研究完整多肽链的解离的模型。

Hemoglobin Tokyo II[α89 (FG1)His-Asn]: A new Hemoglobin Variant Characterized by Endoproteinase Asp-N Digestion and Collision-Induced Dissociation

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