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首页> 外文期刊>Journal of natural fibers >An Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute (Corchorus capsularis L.)
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An Efficient Regeneration System and Optimization of the Transformation from the Cotyledonary Node of Jute (Corchorus capsularis L.)

机译：黄麻（Corchorus capsularis L.）子叶节的高效再生系统和转化的优化。

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摘要

The purpose of this study was to use cotyledonary nodes as explants to establish an efficient regeneration protocol for jute (Corchorus capsularis L.) on Murashige and Skoog (MS) basal medium. Research into the different growth regulators using the orthogonal design L16 (4(5)) revealed that the best shoot induction medium is MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6BA and 0.25 mg/L NAA. The average number of shoots per explant and the explant induction rate were 9.8 and 100%. After 3 weeks, 2-3 cm shoots were rooting on 1/2 MS medium containing 8% (w/v) agar and 3% (w/v) sucrose supplemented with 1.0 mg/L 6-BA + 0.2 mg/L NAA. Moreover, we optimized Agrobacterium-mediated transformation using the GUS gene transient expression system. The best condition for obtaining higher transformation rate consisted of the use of fresh explants to which 100 M acetosyringone was added for a co-culture time of 10 min, the OD value of Agrobacterium liquid is 0.5 at 600 nm. These data provide an important basis for the application of other trait gene in the improvement of jute fiber quality.
机译：这项研究的目的是使用子叶节作为外植体，以在Murashige和Skoog（MS）基础培养基上建立黄麻（Corchorus荚risL。）的有效再生方案。使用正交设计L16（4（5））对不同生长调节剂的研究表明，最佳芽诱导培养基是MS培养基，其中包含8％（w / v）琼脂和3％（w / v）蔗糖，并补充1.0 mg / L 6BA和0.25 mg / L NAA。每个外植体的平均芽数和外植体诱导率分别为9.8和100％。 3周后，将2-3 cm芽生根于1/2 MS培养基中，该培养基含有8％（w / v）琼脂和3％（w / v）蔗糖，并辅以1.0 mg / L 6-BA + 0.2 mg / L NAA 。此外，我们使用GUS基因瞬时表达系统优化了农杆菌介导的转化。获得更高转化率的最佳条件是使用新鲜的外植体，在其中加入100 M乙酰丁香酮，共培养10分钟，在600 nm处农杆菌液的OD值为0.5。这些数据为应用其他性状基因改善黄麻纤维品质提供了重要依据。

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  • 来源
    《Journal of natural fibers》 |2015年第4期|303-310|共8页
  • 作者

    Zhang Gaoyang; Zhang Yujia; Xu Jiantang; Li Feng Tao; Tao Aifen; Zhang Liwu; Fang Pingping; Lin Lihui; Qi Jianmin;

  • 作者单位

    Shangrao Normal Univ, Coll Life Sci, Shangrao, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

    Fujian Agr & Forestry Univ, Key Lab Genet Breeding & Multiple Utilizat Crops, Fuzhou 350002, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Agrobacterium; cotyledonary node; fiber crop; regeneration; Transformation; Acetosyringone;

    机译：农杆菌;子叶节点;纤维作物;再生;转化;乙酰丁香酮;

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