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首页> 外文期刊>Review of Scientific Instruments >Design and demonstration of multimodal optical scanning microscopy for confocal and two-photon imaging
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Design and demonstration of multimodal optical scanning microscopy for confocal and two-photon imaging

机译：共模和双光子成像的多峰光学扫描显微镜的设计和演示

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摘要

We developed a multimodal microscopy based on an optical scanning system in order to obtain diverse optical information of the same area of a sample. Multimodal imaging researches have mostly depended on a commercial microscope platform, easy to use but restrictive to extend imaging modalities. In this work, the beam scanning optics, especially including a relay lens, was customized to transfer broadband (400–1000 nm) lights to a sample without any optical error or loss. The customized scanning optics guarantees the best performances of imaging techniques utilizing the lights within the design wavelength. Confocal reflection, confocal fluorescence, and two-photon excitation fluorescence images were obtained, through respective implemented imaging channels, to demonstrate imaging feasibility for near-UV, visible, near-IR continuous light, and pulsed light in the scanning optics. The imaging performances for spatial resolution and image contrast were verified experimentally; the results were satisfactory in comparison with theoretical results. The advantages of customization, containing low cost, outstanding combining ability and diverse applications, will contribute to vitalize multimodal imaging researches.
机译：我们开发了基于光学扫描系统的多峰显微镜，以获取样品同一区域的多种光学信息。多峰成像研究主要依赖于商用显微镜平台，该平台易于使用，但在扩展成像方式方面受到限制。在这项工作中，定制了光束扫描光学器件，尤其是包括中继透镜的器件，以将宽带（400–1000 nm）的光传输到样品，而没有任何光学误差或损失。定制的扫描光学器件可利用设计波长内的光确保最佳成像技术性能。通过各自实现的成像通道获得了共聚焦反射，共聚焦荧光和双光子激发荧光图像，以证明在扫描光学系统中近紫外，可见，近红外连续光和脉冲光的成像可行性。实验验证了空间分辨率和图像对比度的成像性能；与理论结果相比，结果令人满意。定制的优势包括低成本，出色的组合能力和多样化的应用，将有助于振兴多模态成像研究。

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