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首页> 外文期刊>Russian journal of marine biology >Deoxyribonucleases (DNases) in the Cortex and Endosome from the Marine Sponge Tethya aurantium
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Deoxyribonucleases (DNases) in the Cortex and Endosome from the Marine Sponge Tethya aurantium

机译：海洋海绵Tethya aurantium的皮质和内体中的脱氧核糖核酸酶（DNases）

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摘要

The presence and activity of deoxyribonucleases in the cortex and endosome sections from a sponge, the sea orange Tethya aurantium, were investigated. The maximal enzyme activity in sponge homo-genate was detected at pH 4.27, pH 7.0 and pH 8.5-8.75. Among different specimens, several distinct patterns of neutral DNase isozymes were observed in the cortex section. In each investigated specimen the highest neutral DNase activity belonged to high molecular weight proteins (up to75 kDa). The acid DNases showed a low level of enzyme activity. In the endosome section the acid DNase activity was up to ten times higher than in the cortex and the presence of DNase Ⅱ-like protein was detected. Neutral DNase, which expressed the highest enzyme activity in all the investigated specimens, has a molecular weight of 20 kDa and belongs to the DNase I-like family. The results indicate that the activity of neutral and acid DNases is related to sponge sections and their biological functions. The cortex, as the sponge section that communicates with the environment, expresses high interindividual variability and heterogeneity of neutral DNases, while the endosome section, where the intracellular digestion is localized, is a site of high acid DNase activity.
机译：研究了海绵（海橙Tethya aurantium）的皮质和内体部分中脱氧核糖核酸酶的存在和活性。在pH 4.27，pH 7.0和pH 8.5-8.75下检测到海绵匀浆中的最大酶活性。在不同的标本中，在皮质部分观察到几种不同的中性DNase同工酶模式。在每个调查的样本中，最高的中性DNase活性属于高分子量蛋白质（最高75 kDa）。酸性DNA酶显示出低水平的酶活性。在核内体部分，酸性DNase活性比皮质高十倍，并且检测到存在DNaseⅡ-样蛋白。在所有研究样本中表达最高酶活性的中性DNase分子量为20 kDa，属于DNase I样家族。结果表明，中性和酸性DNA酶的活性与海绵切片及其生物学功能有关。皮质是与环境沟通的海绵部分，在个体间表现出高度的变异性和中性DNase的异质性，而胞内消化所在的内体部分则是高酸性DNase活性的位点。

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