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首页> 外文期刊>Science in China. Series C, Life sciences >Enhancing disease resistances of Super Hybrid Rice with four antifungal genes
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Enhancing disease resistances of Super Hybrid Rice with four antifungal genes

机译：利用四个抗真菌基因提高超级杂交稻的抗病性

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摘要

A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of '9311', an indica restorer line of Super Hybrid Rice, via modified biolistic particle bombardment. Southern blot analysis indicated that in the regenerated hygromycin-resistant plants, all the four antifungal genes, including RCH10, RAC22, β-Glu and B-RIP, were integrated into the genome of '9311', co-transmitted altogether with the marker gene hpt in a Mendelian pattern. Some transgenic R_1 and R_2 progenies, with all transgenes displaying a normal expression level in the Northern blot analysis, showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively. Furthermore, transgenic F_1 plants, resulting from a cross of R_2 homozygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S, showed not only high resistance to M. grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).
机译：通过修饰的生物弹丸轰击，将具有四个抗真菌基因的植物表达载体递送到超级杂交稻的restore恢复系'9311'的胚发生愈伤组织中。 Southern印迹分析表明，在再生的潮霉素抗性植物中，包括RCH10，RAC22，β-Glu和B-RIP在内的四个抗真菌基因都整合到了'9311'基因组中，并与标记基因一起共同传播。孟德尔模式中的hpt。一些R_1和R_2转基因子代，在Northern印迹分析中所有转基因均显示正常表达水平，当分别在位于雁西和三亚的典型高产育苗场中进行测试时，它们对稻瘟病菌显示出高抗性。此外，由R_2纯合品系与非转基因雄性不育系Peiai 64S杂交而成的具有高抗稻瘟病性的R_2纯合品系不仅产生了对稻瘟病菌的高抗性，而且还增强了对水稻假黑穗病的抗性（a稻瘟病和稻粒黑穗病（巴氏丝虫病引起的另一种疾病）。

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