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首页> 外文期刊>Science in China, Series C. Life science >Enhancing disease resistances of Super Hybrid Rice with four antifungal genes
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Enhancing disease resistances of Super Hybrid Rice with four antifungal genes

机译:利用四个抗真菌基因增强超级杂交稻的抗病性

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摘要

A plant expression vector harboring four antifungal genes was delivered into the embryogenic calli of '9311',an indica restorer line of Super Hybrid Rice,via modified biolistic particle bombardment.Southern blot analysis indicated that in the regenerated hygromycin-resistant plants,all the four antifungal genes,including RCH10,RAC22,beta-Glu and B-RIP,were integrated into the genome of '9311',co-transmitted altogether with the marker gene hpt in a Mendelian pattern.Some transgenic R_1 and R_2 progenies,with all transgenes displaying a normal expression level in the Northern blot analysis,showed high resistance to Magnaporthe grisea when tested in the typical blast nurseries located in Yanxi and Sanya respectively.Furthermore,transgenic F_1 plants,resulting from a cross of R_2 homozygous lines with high resistance to rice blast with the non-transgenic male sterile line Peiai 64S,showed not only high resistance to M.grisea but also enhanced resistance to rice false smut (a disease caused by Ustilaginoidea virens) and rice kernel smut (another disease caused by Tilletia barclayana).
机译:通过修饰的基因枪轰击,将具有四个抗真菌基因的植物表达载体转移到超级杂交水稻的restore恢复系'9311'的胚发生愈伤组织中。Southern印迹分析表明,在再生的潮霉素抗性植物中,所有这四个将包括RCH10,RAC22,β-Glu和B-RIP在内的抗真菌基因整合到'9311'的基因组中,与标记基因hpt一起以孟德尔模式共同传播。一些R_1和R_2转基因子代,所有转基因在北部杂交分析中显示出正常的表达水平,在分别位于雁西和三亚的典型高产苗圃中进行试验时,对稻瘟病菌具有较高的抗性。此外,转基因的F_1植物是由R_2纯合品系杂交而成,对水稻具有高抗性用非转基因雄性不育系Peiai 64S进行稻瘟病,不仅显示出对稻瘟病菌的高抗性,而且还增强了对水稻假黑穗病的抵抗力稻瘟病菌(Ustilaginoidea virens)和稻仁黑穗病(Tilletia barclayana引起的另一种疾病)。

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