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首页> 外文期刊>Science in China. Series C, Life sciences >Heat shock response and mammal adaptation to high elevation (hypoxia)
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Heat shock response and mammal adaptation to high elevation (hypoxia)

机译：热休克反应和哺乳动物适应高海拔（缺氧）

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The mammal's high elevation (hypoxia) adaptation was studied by using the immu-nological and the molecular biological methods to understand the significance of Hsp (hypoxia) adaptation in the organic high elevation, through the mammal heat shock response. (1) From high elevation to low elevation (natural hypoxia): Western blot and conventional RT-PCR and real-time fluorescence quota PCR were adopted. Expression difference of heat shock protein of 70 (Hsp70) and natural expression of brain tissue of Hsp70 gene was determined in the cardiac muscle tissue among the different elevation mammals (yak). (2)From low elevation to high elevation (hypoxia induction): The mammals (domestic rabbits) from the low elevation were sent directly to the areas with different high elevations like 2300, 3300 and 5000 m above sea level to be raised for a period of 3 weeks before being slaughtered and the genetic inductive expression of the brain tissue of Hsp70 was determined with RT-PCR. The result indicated that all ofthe mammals at different elevations possessed their heat shock response gene. Hsp70 of the high elevation mammal rose abruptly under stress and might be induced to come into being by high elevation (hypoxia). The speedy synthesis of Hsp70 in the processof heat shock response is suitable to maintain the cells' normal physiological functions under stress. The Hsp70 has its threshold value. The altitude of 5000 m above sea level is the best condition for the heat shock response, and it starts to reduce when the altitude is over 6000 m above sea level. The Hsp70 production quantity and the cell hypoxia bearing capacity have their direct ratio.
机译：通过使用免疫学和分子生物学方法研究哺乳动物的高海拔（低氧）适应性，以通过哺乳动物的热休克反应了解Hsp（低氧）适应性在有机高海拔中的重要性。 （1）从高海拔到低海拔（自然缺氧）：采用蛋白质印迹和常规RT-PCR以及实时荧光定量PCR。在不同海拔的哺乳动物（y牛）中，测定了心肌组织中热休克蛋白70（Hsp70）的表达差异和Hsp70基因的脑组织的自然表达。 （2）从低海拔到高海拔（低氧诱导）：低海拔的哺乳动物（家兔）被直接送入海拔2300、3300和5000 m的不同海拔高的区域，并进行一段时间的饲养。宰杀前3周，用RT-PCR测定Hsp70脑组织的遗传诱导表达。结果表明，不同海拔高度的所有哺乳动物均具有热休克反应基因。高海拔哺乳动物的Hsp70在压力下突然上升，可能被高海拔引起（缺氧）。热休克反应过程中Hsp70的快速合成适合在压力下维持细胞的正常生理功能。 Hsp70具有其阈值。海拔5000 m的高度是热冲击响应的最佳条件，当海拔超过6000 m时，海拔开始降低。 Hsp70的产量和细胞缺氧的能力直接相关。

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