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Optimization of fluorescent cell-based assays for high-throughput analysis using microchamber array chip formats

机译：使用微腔阵列芯片格式优化用于高通量分析的基于荧光细胞的测定

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This study describes the optimization of fluorescent cell-based assays using microchamber array chip formats as well as using automatic nanoliter volumes of sample dispensing system for high-throughput screening analysis of anticancer drugs. Cell-based assays can be employed efficiently in the screening of potential anticancer drug candidates and bioactive compounds with distinct biological function. Identification and development of cell-based assays adapted to high-throughput screening requirements is important when screening chemicals for their potential anticancer properties. Cell-based screening assays using microchamber array chip formats and automatic nanoliter volumes of sample dispensing system requires an optimization as a prerequisite for parameters including assay liquid volume and number of cells per each chamber, and the total cell-based assay itself. Further, the anticancer effect of mitomycin C was studied as an example against human cervical carcinoma cell line-HeLa 229 using cell-based assay that was optimized on chamber array chip formats and determined the cytotoxicity of mitomycin C by measuring the cell proliferation of HeLa with Calcein-AM fluorescent dye. The cell-based screening assay that was performed using chamber array chip formats was compared with the conventional 96-well plate formats was discussed. The assay described in this study is rapid, simple and inexpensive that is desirable in selecting anticancer candidates.
机译：这项研究描述了使用微腔阵列芯片格式以及自动纳升体积的样品分配系统进行的基于荧光细胞的检测方法的优化，以进行抗癌药物的高通量筛选分析。基于细胞的分析可以有效地用于筛选潜在的抗癌药物候选物和具有独特生物学功能的生物活性化合物。在筛选化学药品的潜在抗癌特性时，识别和开发适合高通量筛选要求的基于细胞的测定非常重要。使用微室阵列芯片格式和自动纳升体积的样品分配系统进行的基于细胞的筛选测定需要进行优化，作为参数的前提条件，包括测定液体体积和每个小室的细胞数以及基于细胞的总测定本身。此外，以基于细胞的测定法研究了丝裂霉素C对人宫颈癌细胞系HeLa 229的抗癌作用，对细胞阵列芯片格式进行了优化，并通过测量HeLa的细胞增殖来确定丝裂霉素C的细胞毒性。钙黄绿素-AM荧光染料。讨论了使用室阵列芯片形式与常规96孔板形式进行的基于细胞的筛选测定。这项研究中描述的测定是快速，简单和廉价的，这在选择抗癌候选物中是理想的。

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