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首页> 外文期刊>The Science of the Total Environment >Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol)
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Effect of long-term manure slurry application on the occurrence of antibiotic resistance genes in arable purple soil (entisol)

机译：长期施用粪肥对紫色耕地（ENTSOL）抗生素抗性基因发生的影响

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摘要

The application of animal manure is a highly recommended traditional agricultural practice for soils of relatively low fertility. However, for the farmland purple soils that are widely distributed in the upper Yangtze River region, little knowledge has been established in previous studies about the changes in the antibiotic resistome upon manure amendment. In the present study, the impact of long-term pig manure slurry application on the occurrence of antibiotic resistance genes (ARGs) and bacterial community was assessed in arable calcareous purple soil using high-throughput quantitative polymerase chain reaction and Illumina sequencing. Four treatments, including a non-fertilization control (CK) and pig manure (OM), OM plus mineral N fertilizer (OMN) and OM plus mineral NPK fertilizer (OMNPK) treatments were investigated. Across all the soil samples receiving different treatments, a total of 139 unique ARGs and 6 mobile genetic element genes were detected, with multidrug and beta-lactam the two most dominant types of ARGs. The results of the principal coordinate analysis (PCoA) suggest that the profiles of soil ARGs in the two treatments of OM combined with mineral fertilizer(s) (i.e., OMN and OMNPK) were similar to those in the control treatment, while the soil receiving only pig manure application had a different pattern of ARGs from the soils in the other three treatments. A clear reduction of soil ARGs was observed in the OM treatment. Significant and positive relationships were found not only among ARGs but also between mobile genetic elements (MGEs) and ARGs. However, no significant relationships were detected between ARG patterns and bacterial community composition. These results imply that the long-term application of pig manure slurry to purple soil does not lead to the prevalence of ARGs; however, the potential for the horizontal transfer of ARGs in calcareous purple soil should not be ignored. (C) 2018 Elsevier B.V. All rights reserved.
机译：强烈建议在肥力相对较低的土壤中使用动物肥料。然而，对于长江上游地区广泛分布的农田紫色土壤，以前的研究对粪便改良后抗生素抗药性的变化知之甚少。在本研究中，使用高通量定量聚合酶链反应和Illumina测序，评估了长期施用猪粪浆对紫色石灰性土壤中抗生素抗性基因（ARGs）和细菌群落发生的影响。研究了四种处理方法，包括非施肥控制（CK）和猪粪（OM），OM加矿物氮肥（OMN）和OM加矿物氮磷钾肥（OMNPK）。在接受不同处理的所有土壤样品中，共检测到139个独特的ARG和6个流动遗传元素基因，其中多药和β-内酰胺是两种最主要的ARG类型。主坐标分析（PCoA）的结果表明，两种有机肥与矿物肥料（即OMN和OMNPK）联合处理的两种土壤中的土壤ARG的分布与对照处理中的相似，而土壤接受在其他三种处理中，只有猪粪施用的ARGs模式与土壤不同。在OM处理中观察到土壤ARGs明显减少。不仅在ARG之间而且在移动遗传元件（MGE）和ARG之间都发现了显着和积极的关系。但是，在ARG模式和细菌群落组成之间未发现明显的关系。这些结果表明，在紫色土壤上长期施用猪粪浆不会导致ARGs的流行。但是，在钙质紫色土中ARGs水平转移的潜力不容忽视。 （C）2018 Elsevier B.V.保留所有权利。

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