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首页> 外文期刊>Science of the total environment >Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology
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Remediation of petroleum hydrocarbon-contaminated sites by DNA diagnosis-based bioslurping technology

机译：通过基于DNA诊断的生物制浆技术修复石油烃污染场所

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摘要

The application of effective remediation technologies can benefit from adequate preliminary testing, such as in lab-scale and Pilot-scale systems. Bioremediation technologies have demonstrated tremendous potential with regards to cost, but they cannot be used for all contaminated sites due to limitations in biological activity. The purpose of this study was to develop a DNA diagnostic method that reduces the time to select contaminated sites that are good candidates for bioremediation. We applied an oligonucleotide microarray method to detect and monitor genes that lead to aliphatic and aromatic degradation. Further, the bioremediation of a contaminated site, selected based on the results of the genetic diagnostic method, was achieved successfully by applying bioslurping in field tests. This gene-based diagnostic technique is a powerful tool to evaluate the potential for bioremediation in petroleum hydrocarbon contaminated soil.
机译：有效的补救技术的应用可以从适当的初步测试中受益，例如在实验室规模和中试规模的系统中。生物修复技术在成本方面已显示出巨大的潜力，但由于生物活性的限制，它们不能用于所有受污染的地点。这项研究的目的是开发一种DNA诊断方法，以减少选择受污染部位的时间，这些部位是进行生物修复的良好候选者。我们应用了一种寡核苷酸微阵列方法来检测和监测导致脂肪族和芳香族降解的基因。此外，基于遗传诊断方法的结果选择的污染部位的生物修复是通过在现场测试中应用生物制浆技术成功实现的。这种基于基因的诊断技术是评估石油烃污染土壤中生物修复潜力的有力工具。

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  • 来源
    《Science of the total environment》 |2014年第1期|250-259|共10页
  • 作者

    Seungjin Kim; Rosa Krajmalnik-Brown; Jong-Oh Kim; Jinwook Chung;

  • 作者单位

    Environmental Engineering Department, Samsung Engineering Co., Ltd., 500 Samsung GEC, Sangil-Dong, Gangdong-Gu, Seoul 134-728, Republic of Korea;

    Center for Environmental Biotechnology, Biodesign Institute at Arizona State University, 1001 South McAllister Avenue, Tempe, AZ 85287-5701, USA;

    Department of Civil and Environmental Engineering, Hanyang University, 222 Wangsimni-Ro, Seongdong-Gu, Seoul 133-791, Republic of Korea;

    R&D Center, Samsung Engineering Co., Ltd., 415-10 Woncheon-Dong, Youngtong-Gu, Suwon, Gyeonggi-Do 443-823, Republic of Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bioslurping; BTEX; Diagnosis technology; Oligonucleotide microarray; TPH;

    机译：生物浆化;BTEX;诊断技术;寡核苷酸微阵列;TPH;

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