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首页> 外文期刊>Science of the total environment >Phenotypic antibiotic resistance of Escherichia coli and E. coli 0157 isolated from water, sediment and biofilms in an agricultural watershed in British Columbia
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Phenotypic antibiotic resistance of Escherichia coli and E. coli 0157 isolated from water, sediment and biofilms in an agricultural watershed in British Columbia

机译：从不列颠哥伦比亚省一个农业流域的水，沉积物和生物膜中分离出的大肠杆菌和大肠杆菌0157的表型抗生素抗性

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摘要

This study examined the distribution of antibiotic resistant Escherichia coli and E. coli 0157 isolated from water, sediment and biofilms in an intensive agricultural watershed (Elk Creek, British Columbia) between 2005 and 2007. It also examined physical and chemical water parameters associated with antibiotic resistance. Broth microdilution techniques were used to determine minimum inhibitory concentrations (MIC) for E. coli (n = 214) and E. coli 0157 (n = 27) recovered isolates for ampicillin, cefotaxsme, ciprofloxacin, nalidixic acid, streptomycin and tetracycline. Both E. coli and E. coli 0157 isolates showed highest frequency of resistance to tetracycline, ampicillin, streptomycin and nalidixic acid; respectively. For E. coli, the highest frequency of resistance was observed at the most agriculturally-impacted site, while the lowest frequency of resistance was found at the headwaters. Sediment and river rock biofilms were the most likely to be associated with resistant E. coli, while water was the least likely. While seasonality (wet versus dry) had no relationship with resistance frequency, length of biofilm colonization of the substratum in the aquatic environment only affected resistance frequency to nalidixic acid and tetracycline. Multivariate logistic regressions showed that water depth, nutrient concentrations, temperature, dissolved oxygen and salinity had statistically significant associations with frequency of E. coli resistance to nalidixic acid, streptomycin, ampicillin and tetracycline. The results indicate that antibiotic resistant E. coli and E. coli 0157 were prevalent in an agricultural stream. Since E. coli is adept at horizontal gene transfer and prevalent in biofilms and sediment, where ample opportunities for genetic exchange with potential environmental pathogens present themselves, resistant isolates may present a risk to ecosystem, wildlife and public health.
机译：这项研究检查了2005年至2007年之间在集约化农业流域（不列颠哥伦比亚省埃克克里克）从水，沉积物和生物膜中分离出的抗药性大肠杆菌和大肠杆菌0157的分布。还研究了与抗生素有关的物理和化学水参数抵抗性。肉汤微稀释技术用于确定对氨苄青霉素，头孢噻肟，环丙沙星，萘啶酸，链霉素和四环素的大肠杆菌（n = 214）和大肠杆菌0157（n = 27）回收菌株的最低抑菌浓度（MIC）。大肠杆菌和大肠杆菌0157分离株均显示出对四环素，氨苄青霉素，链霉素和萘啶酸的最高抗药性。分别。对于大肠杆菌，在受农业影响最大的地点观察到最高的抗药性频率，而在上游源头发现的最低抗药性频率。沉积物和河流岩石生物膜最可能与抗性大肠杆菌有关，而水则最不相关。虽然季节性（干湿对干）与抗性频率没有关系，但是在水生环境中基质生物膜定殖的长度仅影响耐萘啶酸和四环素的抗性频率。多元logistic回归显示，水深，营养物浓度，温度，溶解氧和盐度与大肠杆菌对萘啶酸，链霉素，氨苄青霉素和四环素的耐药性频率具有统计学上的显着关联。结果表明，抗生素抗性大肠杆菌和大肠杆菌0157在农业生产中普遍存在。由于大肠杆菌擅长水平基因转移，并在生物膜和沉积物中普遍存在，因此存在与潜在环境病原体进行遗传交换的充分机会，因此耐药菌株可能会对生态系统，野生动植物和公共健康构成威胁。

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  • 来源
    《Science of the total environment》 |2013年第15期|315-323|共9页
  • 作者

    Rasha Maal-Bared; Karen H. Bartlett; William R. Bowie; Eric R. Hall;

  • 作者单位

    Resource Management and Environmental Studies, University of British Columbia, Vancouver, Canada,Centre for Research on Environmental Microbiology, University of Ottawa, 451 Smyth Road, Ottawa. Ontario, Canada K1H 8M5;

    School of Environment and Health, 2206 East Mall, University of British Columbia, Vancouver, BC, Canada V6Z 1Z3;

    University of British Columbia, Division of Infectious Diseases, 452D Heather Pavilion East, Vancouver General Hospital, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5;

    Department of Civil Engineering, 6250 Applied Science Lane, The University of British Columbia, Vancouver, BC, Canada V6Z 1Z4;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    biofilms; sediment; minimum inhibitory concentration (MIC); E. coli 0157; multiple antibiotic resistance (MAR);

    机译：生物膜沉淀;最低抑菌浓度（MIC）大肠杆菌0157;多种抗生素耐药性（MAR）;

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