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首页> 外文期刊>The Science of the Total Environment >Accurately quantifying the reductive capacity of microbial extracellular polymeric substance by mediated electrochemical oxidation method
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Accurately quantifying the reductive capacity of microbial extracellular polymeric substance by mediated electrochemical oxidation method

机译:通过介导的电化学氧化方法准确地量化微生物细胞外聚合物物质的还原能力

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摘要

The reductive capacity of microbial extracellular polymeric substances (EPS) plays important roles in environmental processes involved in heavy metal detoxification and organic contaminant degradation. However, the crucial parameter to evaluate the reductive capacity of EPS, electron donating capacity (EDC) lacks a quantitative approach. In this study, a novel mediated electrochemical oxidation (MEO) method was developed to investigate the EDCs of microbial EPS extracted fromSliewanella oneidensis MR-1 (S. oneidensis MR-1), Escheichia colt E. coil) and activated sludge. The results indicate that the MEO approach rapidly and accurately quantifies the EDCs of microbial EPS. S. oneidensis MR-1 EPS possessed the highest EDC value ascribed to their specific redox proteins components. EDCs of S. oneidensis MR-1 EPS were dependent on measurement conditions and increased with growing solution pH and applied potential. EDCs of S. oneidensis MR-1 EPS were depleted gradually during the redox reaction with irreversible oxidation of EPS. The reductive property of microbial EPS was accurately evaluated by quantifying the EDCs of EPS using the MEO approach, as well as their potential in environmental remediation. (C) 2019 Elsevier B.V. All rights reserved.
机译:微生物细胞外聚合物物质(EPS)的还原能力在涉及重金属排毒和有机污染物降解中的环境过程中起重要作用。然而,评估EPS的还原能力的关键参数,电子捐赠容量(EDC)缺乏定量方法。在该研究中,开发了一种新型介导的电化学氧化(MEO)方法以研究从Sliewanella onidensis MR-1(Iidensis MR-1),Escheichia Colt E.线圈)和活性污泥中提取的微生物EPS的EDC。结果表明,MEO迅速且准确地量化了微生物EPS的EDC。 S. onidensis MR-1 EPS具有归因于其特定的氧化还原蛋白组分的最高EDC值。 S.Inidensis MR-1 EPS的EDC依赖于测量条件并随着溶液pH的生长和应用潜力而增加。在氧化还原反应期间逐渐耗尽了S. onidensis MR-1 EP的EDCS与EPS不可逆的氧化。通过使用MEO方法量化EPS的EDC,以及它们在环境修复中的潜力来精确评估微生物EPS的还原性能。 (c)2019 Elsevier B.v.保留所有权利。

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