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首页> 外文期刊>Science of the total environment >Using a next-generation sequencing approach to DNA metabarcoding for identification of adulteration and potential sources of mercury in commercial cat and dog foods
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Using a next-generation sequencing approach to DNA metabarcoding for identification of adulteration and potential sources of mercury in commercial cat and dog foods

机译：使用下一代测序方法进行DNA地区识别商业猫和狗食品中掺假和潜在来源的DNA地区

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Studies have demonstrated that some commercial pet (i.e., cat and dog) food products contain high concentrations of mercury (Hg), and some products have Hg concentrations that are higher than expected based on the ingredients included in the package ingredient list. Additionally, concentrations of methylmercury, a particularly toxic form of Hg commonly associated with fish-based ingredients, are largely unstudied despite the widespread use of such ingredients in pet food products. This study aimed to quantify total Hg and methylmercury in a variety of commercial pet food products (n = 127), and use genetic tools to determine if specific ingredients contributed to high Hg concentrations in the final product. Results indicate that total Hg concentrations were above suggested maximum tolerable limits in three of the tested pet food products, and that methylmercury concentrations were at safe levels in all tested products. Next-generation amplicon sequencing using ten barcode primers was conducted to target distinct taxa and to determine if one primer set outperformed the others in amplifying the often heavily degraded DNA found in pet food products. The 16sUniF_16sUniR primer set generated a relatively higher number of reads across the broadest set of taxa, although several of the primer sets were useful in identifying common animal- and plant-based ingredients in commercial pet food products. Combined with the Hg results, it was demonstrated that pet food product ingredients are consistent among and between product lots. However, these results also revealed that adulteration is prevalent in pet food products.
机译：有研究表明，一些商业宠物（例如，猫，狗）食品产品中含有高浓度的汞（Hg），有的产品有汞浓度是基于包中包含的成分表中的成分高于预期。此外，甲基汞浓度，常用的有鱼为主成分有关汞的毒性特别形式，在很大程度上是未研究，尽管在宠物食品中广泛使用这种成分。本研究旨在量化总汞和甲基汞在各种商业宠物食品（N = 127），并使用基因工具，以确定具体成分促成了最终产品的高汞浓度。结果表明，总汞浓度在三个测试的宠物食品进行上述建议的最大可容忍的限度，而甲基汞浓度在所有测试产品的安全水平。下一代扩增使用10个条码引物测序进行瞄准不同的类群，并确定一个引物组优于别人放大往往严重退化的DNA在宠物食品中发现。所述16sUniF_16sUniR引物组所产生的相对较高数目的跨最广泛的分类群的读出，虽然有几个引物对分别为在确定共同动物 - 和在商业宠物食品的基于植物的成分是有用的。与汞结合的结果，它证明了宠物食品成分中和产品批次之间是一致的。然而，这些结果还显示，掺假宠物食品的产品普遍。

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