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Shaping Up

机译:塑造

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摘要

Researchers in fields as diverse as neuroscience, angiogen-esis, and regenerative medicine are discovering that the surfaces their cultured cells grow on may be as important as the media they're grown in. Cells grown in traditional two-dimensional cultures in flasks and wells have different topology, architecture, and viscoelasticity compared with cells grown in vivo. This affects a host of cell parameters, such as morphology, growth, and polarity. Two-dimensional cultures also neglect the role that the extracellular matrix plays in informing a cell's decisions, as well as in providing a physical space in which to form 3-D structures. "Most phenotypes that we're following now are not revealed in 2-D," says Joan Brugge, who studies glandular formation ex vivo at Harvard Medical School.
机译:神经科学,血管生成技术和再生医学等众多领域的研究人员发现,培养细胞生长的表面可能与其生长介质一样重要。在烧瓶和孔中传统二维培养中生长的细胞与体内生长的细胞相比,它们具有不同的拓扑结构,结构和粘弹性。这会影响许多细胞参数,例如形态,生长和极性。二维培养还忽略了细胞外基质在告知细胞决定以及提供形成3D结构的物理空间中所起的作用。哈佛医学院的离体研究腺体形成的琼·布鲁格说:“我们现在关注的大多数表型在二维中并未显示出来。”

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  • 来源
    《Scientist》 |2008年第6期|67|共1页
  • 作者

    Josh P. Roberts;

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