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Quantitative and selective DNA detection with portable personal glucose meter using loop-based DNA competitive hybridization strategy

机译：使用基于环的DNA竞争性杂交策略的便携式个人血糖仪进行定量和选择性DNA检测

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摘要

A portable, quantitative and selective DNA detection biosensor requiring only one step for target recognition and signal reporter generation using a loop-based DNA competitive hybridization assay and personal glucose meter (PGM) was developed. In the presence of target DNA, due to its competitive binding, the invertase-DNA conjugate was released which could be collected with the help of a magnet subsequently. The released invertase-DNA was used to catalyze the hydrolysis of sucrose into glucose with millions of turnovers which was target concentration dependent. We found that there was a linear relationship between the PGM signals and the target DNA concentration in the range of 100 pM to 100 nM with a detection limit of 100 pM. In addition, the sensor exhibits excellent sequence selectivity, being able to differentiate a single mismatch in the target DNA, and excellent anti-interference ability, having almost no effect in serum on the detection performance. The biosensor reported here is easier to operate, owning great potential in point of care testing in environments with limited resources and skilled personnel for rapid and sensitive detection of specific DNA sequence in real biological samples.
机译：开发了一种便携式，定量和选择性的DNA检测生物传感器，只需使用一个步骤即可使用基于环的DNA竞争杂交测定法和个人血糖仪（PGM）进行靶标识别和信号报告子生成。在靶DNA的存在下，由于其竞争性结合，释放了转化酶-DNA结合物，其随后可以在磁体的帮助下收集。释放的转化酶-DNA被用于催化蔗糖水解成葡萄糖，其具有数百万周转，这取决于目标浓度。我们发现PGM信号与目标DNA浓度之间的线性关系在100 pM至100 nM的范围内，检测限为100 pM。另外，该传感器表现出优异的序列选择性，能够区分靶DNA中的单个错配，以及优异的抗干扰能力，在血清中对检测性能几乎没有影响。此处报道的生物传感器更易于操作，在资源有限和技术人员匮乏的环境中，在即时检测方面具有巨大潜力，可以快速，灵敏地检测实际生物样品中的特定DNA序列。

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